E-ISSN 2146-8389
 
2020, Vol: 9, Issue: 1
9 / 1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles
Required files to be uploaded

Author Statement

Original Research (Original Article) 


In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa.

Cited by (4)

Abstract
The methanol extract of Basella alba (MEBa) has been shown to improve male fertility. In order to further understand its action mechanism, its antioxidant effect was investigated in vitro. To this respect, the extract was tested on antioxidant enzyme activities and lipid peroxidation using rat testicular homogenate. Furthermore, the effect of the MEBa on oxidation of β-carotene, reduction of ferric ions and scavenging of free radicals was investigated; in addition, phytochemical analyses were performed. The MEBa slightly reduced diphenyl-picrylhydrazyl (DPPH) radical, and inhibited lipid peroxidation and β-carotene oxidation. The MEBa also reduced ferric ions and stimulated the activities of glutathione reductase, catalase and superoxide dismutase. These findings suggest antioxidant properties of MEBa, that could be attributed to phenolic compounds revealed by phytochemical studies of the extract. The MEBa antioxidant properties may thus sustain its various biological activities, such as improvement of male reproductive function.

Key words: Antioxidant; Basella alba; Enzyme activity; Rat; Testis


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Edouard A. Nantia
Articles by Faustin P. T. Manfo
Articles by Nathalie S. E. Beboy
Articles by Paul F. Moundipa
on Google
on Google Scholar
Article Statistics
 Viewed: 6095
Downloaded: 1051
Cited: 4


REFERENCES
1.Pham-Huy AL, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008; 4:89-96.

[Pubmed]    [PMC Free Fulltext]   


2.Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001; 54:176-86.

http://dx.doi.org/10.1136/jcp.54.3.176

[Pubmed]    [PMC Free Fulltext]   


3.Mathur PP, Saradha B, Vaithinathan S. Impact of environmental toxicants on testicular function. Immun Endocr Metab Agents Med Chem 2008; 8:79-90.

http://dx.doi.org/10.2174/187152208783790705


4.Tiwari AK. Imbalance in antioxidant defense and human diseases: Multiple approach of natural antioxidant therapy. Curr Sci 2001; 8:1179-87.


5.Devasagayam APT, Tilak CJ, Boloor KK, Sane SK, Ghaskadbi SS, Lele DR. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 2004; 52:794-804.

[Pubmed]   


6.Khalaf AN, Shakya KA, Al-Othman A, El-Agbar Z, Farah H. Antioxidant activity of some common plants. Turk J Biol 2008; 32:51-5.


7.Moundipa FP, Kamtchouing P, Koueta N, Tantchou J, Foyang NP, Mbiapo FT. Effects of aqueous extracts of Hibiscus macranthus and Basella alba in mature rat testis function. J Ethnopharmacol 1999; 65(2):133-9.

http://dx.doi.org/10.1016/S0378-8741(98)00207-4


8.Moundipa FP, Beboy ESN, Zelefack F, Ngouela S, Tsamo E, Schill WB, Monsees KT. Effects of Basella alba and Hibiscus macranthus extracts on testosterone production by adult rat and bull Leydig cells. Asian J Androl 2005; 7:411-7.

http://dx.doi.org/10.1111/j.1745-7262.2005.00056.x

[Pubmed]   


9.Moundipa FP, Ngouela S, Kamtchouing P, Tsamo E, Tchouanguep MF, Carreau S. Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices. Asian J Androl 2006; 8:111-4.

http://dx.doi.org/10.1111/j.1745-7262.2006.00057.x

[Pubmed]   


10.Nantia EA, Moundipa PP, Beboy NSE, Monsees TK, Carreau S. Study of the androgenic effect of the methanol extract of Basella alba L. (Basellaceae) on the male rat reproductive function. Andrologie 2007; 17:129-33.

http://dx.doi.org/10.1007/BF03041166


11.Nantia EA, Manfo PF, Beboy NE, Travert C, Carreau S, Monsees TK, Moundipa PF. Effect of methanol extract of Basella alba L. (Basellaceae) on the fecundity and testosterone level in male rats exposed to flutamide in utero. Andrologia 2012; 44:38-45.

http://dx.doi.org/10.1111/j.1439-0272.2010.01104.x

[Pubmed]   


12.Sato T, Nagata M, Engle ML. Evaluation of antioxidant activity of indigenous vegetables from South and Southeast Asia. In Japan International Research Center for Agricultural Sciences (JIRCAS) Research Highlights 2002, Available via http://www.jircas.affrc.go.jp/english/publication/highlights/2002/2002_05.html (Accessed 21 May 2013).


13.Raju M, Varakumar S, Lakshminarayana R, Krishnakantha PT, Baskaran V. Carotenoid composition and vitamin A activity of medicinally important green leafy vegetables. Food Chem 2007; 101:1598-605.

http://dx.doi.org/10.1016/j.foodchem.2006.04.015


14.Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem 2007; 100:1409-18.

http://dx.doi.org/10.1016/j.foodchem.2005.11.032


15.Cao G, Prior RL. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 1998; 44:1309-15.

[Pubmed]   


16.Nantia AE, Travert C, Manfo TFP, Carreau S, Monsees KT, Moundipa FP. Effects of the Methanol Extract of Basella alba L. (Basellaceae) on steroid production in Leydig cells. Int J Mol Sci 2011; 12:376-84.

http://dx.doi.org/10.3390/ijms12010376

[Pubmed]    [PMC Free Fulltext]   


17.Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Food Sci Technol 1995; 28:25-30.

http://dx.doi.org/10.1016/S0023-6438(95)80008-5


18.Miller HE. A simplified method for the evaluation of antioxidant. J Am Oil Chem Soc 1971; 48:91.

http://dx.doi.org/10.1007/BF02635693


19.Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 1996; 239:70-6.

http://dx.doi.org/10.1006/abio.1996.0292

[Pubmed]   


20.Nilsson UA, Olsson LI, Carlin G, Bylund-Fellenius AC. Inhibition of lipid peroxidation by spin labels. J Biol Chem 1989; 264:11131-5.

[Pubmed]   


21.Ikediobi CO, Badisa VL, Ayuk-Takem LT, Latinwo LM, West J. Response of antioxidant enzymes and redox metabolites to cadmium-induced stress in CRL-1439 normal liver cells. Int J Mol Med 2004; 14:87-92.

[Pubmed]   


22.Misra HP, Fridovich I. The generation of superoxide radical during the autoxidation of hemoglobin. J Biol Chem 1972; 247:6960-2.

[Pubmed]   


23.Harborne JB. Phytochemicals Methods, Chapman and Hall, London, UK, pp 49-188, 1973.


24.Trease GE, Evans WC. Pharmacognosy, 13th edition, Bailliere Tinndall, London, UK, 1989.


25.Halliwell B. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol 2006; 141:312-2.

http://dx.doi.org/10.1104/pp.106.077073

[Pubmed]    [PMC Free Fulltext]   


26.Dauqan MAE, Abdullah A, Sani AH. Natural antioxidants, lipid profile, lipid peroxidation, antioxidant enzymes of different vegetable oils. Adv J Food Sci Technol 2011; 3:308-16.


27.Gulcin I, Oktay M, Kirecci E, Kufrevioglu I. Screening of antioxidant and antimicrobial activities of anise (Pimpella anisum L.) seed extracts. Food Chem 2003; 83:371-82.

http://dx.doi.org/10.1016/S0308-8146(03)00098-0


28.Huda-Faujan N, Noriham A, Norrakiah AS, Babji AS. Antioxidative activities of water extracts of some Malaysian herbs. ASEAN Food J 2007; 14:61-6.


29.Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soyabean oil in cyclodextrin emulsion. J Agric Food Chem 1992; 40:945-8.

http://dx.doi.org/10.1021/jf00018a005


30.Nikolova M, Evstatieva L, Nguyen DT. Screening of plant extracts for antioxidant properties. Botanica Serbica 2011; 35:43-48.


31.Donfack JH, Njayou FN, Ngameni B, Tchana A, Chuisseu DP, Finzi VP, Ngadjui TB, Moundipa FP. In vitro hepatoprotective and antioxidant activities of diprenylated isoflavonoids from Erythrina senegalensis (Fabaceae). Asian J Tradit Med 2008; 3:1-7.


32.Henry WL, Chao-Lin K, Wen-Hui Y, Chia-Hsien L, Hong-Zin L. Antioxidant enzymes activity involvement in luteolin-induced human lung squamous carcinoma CH27 cell apoptosis. Eur J Pharmacol 2006; 534:12-8.

http://dx.doi.org/10.1016/j.ejphar.2006.01.021

[Pubmed]   


33.Olawale O, Ikechukwu NEO, Grace TOO, Chidiebere EU. Oxidative stress and superoxide dismutase activity in brain of rats fed with diet containing permethrin. Biokemistri 2008; 20:93-8.


34.Vivek KG, Surendra KS. Plants as natural antioxidants. Natural Product Radiance 2006; 5:326-34.


35.Bagnyukova TV, Storey KB, Lushchak VI. Adaptive response of antioxidant enzymes to catalase inhibition by aminotriazole in goldfish liver and kidney. Comp Biochem Physiol B Biochem Mol Biol 2005; 142:335-41.

http://dx.doi.org/10.1016/j.cbpb.2005.08.003

[Pubmed]   


36.Halliwell B. Oxidative damage, lipid peroxidation, and antioxidant protection in chloroplasts. Chem Phys Lipids 1987; 44:327-40.

http://dx.doi.org/10.1016/0009-3084(87)90056-9


37.Granner KD, Weil PA. Regulation of gene expression. In: Harper's Illustrated Biochemistry, 26th edition, Lange/McGraw-Hill, New York, NY, USA, pp 373-395, 2003.


38.Banday AA, Lokhandwala MF. Loss of biphasic effect on Na/K-ATPase activity by angiotensin II involves defective angiotensin type 1 receptor-nitric oxide signaling. Hypertension 2008; 52:1099-105.

http://dx.doi.org/10.1161/HYPERTENSIONAHA.108.117911

[Pubmed]   


39.Hamden K, Silandre D, Delalande C, El Feki A, Carreau S. Protective effects of estrogens and caloric restriction during aging on various rat testis parameters. Asian J Andrology 2008; 10:837-45.

http://dx.doi.org/10.1111/j.1745-7262.2008.00430.x

[Pubmed]   


40.Sathishkumar R, Lakshmi PTV, Annamalai A. Comparative analyses of non enzymatic and enzymatic antioxidants of Enicostemma littorale blume. Int J Pharma Bio Sci 2010; 1:1-16.

This Article Cited By the following articles

The Influence of Surfactant on Decomposition of Pigment Derived from <i>Basella Alba</i> from Fukuoka Prefecture by Heating or Artificial Sunlight Irradiation
Journal of the Mining and Materials Processing Institute of Japan 2017; 133(5): 92.

1
 
The role of wild fruits and vegetables in delivering a balanced and healthy diet
Food Research International 2017; 99(): 15.

2
 
Protective effect of Basella alba and Carpolobia alba extracts against maneb-induced male infertility.
Pharmaceutical biology 2014; 52(1): 97-104.

3
 

2014; (): 323.

4
 
How to Cite this Article
Pubmed Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxid Antioxid Med Sci. 2013; 2(2): 131-136. doi:10.5455/oams.100413.or.037

Web Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. http://www.ejmoams.com/?mno=33691 [Access: February 16, 2020]. doi:10.5455/oams.100413.or.037

AMA (American Medical Association) Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxid Antioxid Med Sci. 2013; 2(2): 131-136. doi:10.5455/oams.100413.or.037


Vancouver/ICMJE Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxid Antioxid Med Sci. (2013), [cited February 16, 2020]; 2(2): 131-136. doi:10.5455/oams.100413.or.037


Harvard Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa (2013) In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxid Antioxid Med Sci, 2 (2), 131-136. doi:10.5455/oams.100413.or.037


Turabian Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. 2013. In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxidants and Antioxidants in Medical Science, 2 (2), 131-136. doi:10.5455/oams.100413.or.037


Chicago Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. "In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate." Oxidants and Antioxidants in Medical Science 2 (2013), 131-136. doi:10.5455/oams.100413.or.037


MLA (The Modern Language Association) Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa. "In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate." Oxidants and Antioxidants in Medical Science 2.2 (2013), 131-136. Print. doi:10.5455/oams.100413.or.037


APA (American Psychological Association) Style

Edouard A. Nantia, Faustin P. T. Manfo, Nathalie S. E. Beboy, Paul F. Moundipa (2013) In vitro antioxidant activity of the methanol extract of Basella alba L (Basellaceae) in rat testicular homogenate. Oxidants and Antioxidants in Medical Science, 2 (2), 131-136. doi:10.5455/oams.100413.or.037




Most Viewed Articles
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Effects of silymarin, N-acetylcysteine and selenium in the treatment of papulopustular acne
    Haidar Hamid Al-Anbari, Ahmed Salih Sahib, Ahmed R. Abu Raghif
    Oxid Antioxid Med Sci. 2012; 1(3): 201-207
    » Abstract & References » doi: 10.5455/oams.290912.or.019

    •
    Most Downloaded
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica
    Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano
    Oxid Antioxid Med Sci. 2015; 4(1): 49-55
    » Abstract & References » doi: 10.5455/oams.180415.or.084

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Storage lesions in blood components
    Rajashekharaiah Vani, Ravikumar Soumya, Krishnegowda Manasa, Hsieh Carl
    Oxid Antioxid Med Sci. 2015; 4(3): 125-132
    » Abstract & References » doi: 10.5455/oams.130915.rv.019

    •
    Most Cited Articles
  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009
    Cited : 14 times [Click to see citing articles]

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038
    Cited : 14 times [Click to see citing articles]

  • Silymarin and naringenin protects nicotine induced oxidative stress in young rats
    Anshu Jain, Nidhi Dwivedi, Rakesh Bhargava, Swaran J.S. Flora
    Oxid Antioxid Med Sci. 2012; 1(1): 41-49
    » Abstract & References » doi: 10.5455/oams.130412.or.004
    Cited : 13 times [Click to see citing articles]

  • Role of free radicals and antioxidants in gynecological cancers: current status and future prospects
    Lokanatha Valluru, Subramanyam Dasari, Rajendra Wudayagiri
    Oxid Antioxid Med Sci. 2014; 3(1): 15-26
    » Abstract & References » doi: 10.5455/oams.201113.rv.011
    Cited : 13 times [Click to see citing articles]

  • The potential role of S-allylcysteine as antioxidant against various disorders in animal models
    V. V. Sathibabu Uddandrao, Parim Brahmanaidu, Balaji Meriga, Ganapathy Saravanan
    Oxid Antioxid Med Sci. 2016; 5(3): 79-86
    » Abstract » doi: 10.5455/oams.240716.rv.025
    Cited : 11 times [Click to see citing articles]

    •
    This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.
    The articles in Oxidants and Antioxidants in Medical Science are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
    Copyright © 2020 Oxidants and Antioxidants in Medical Science All Rights Reserved. Subject to change without notice from or liability to Oxidants and Antioxidants in Medical Science.
    For best results, please use Internet Explorer or Google Chrome

    Contact Information


    Your comments are very important to us

    We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below.
     
    Email us at info[at]scopemed.com
     
    We will respond within 48 to 72 hours.
     
    Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publishing Ethics
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use