ISSN 2146-8389
 

Original Research (Original Article) 


Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts

Yudi Purnomo, Yvette Piccart, Tamara Coenen, Jos van Pelt, John S. Prihadi, Paul J. Lijnen.

Cited by (1)

Abstract
The aim of the present study was to determine whether transforming growth factor-β1 (TGF-β1)-induced collagen production in cardiac fibroblasts is affected by reactive oxygen species (ROS). Cardiac fibroblasts (passage 2) from normal male adult rats were cultured to confluency and incubated in serum-free Dulbecco’s modified Eagle’s medium for 24 h. The cells were then preincubated with(out) the tested inhibitors for 1 h and further incubated with(out) TGF-β1 at various concentrations and for 1, 2, 4, 24 or 48 h. TGF-β1 induced a dose-dependent increase in the soluble collagen production in cardiac fibroblasts after 48 h of incubation. No significant effect of TGF-β1 (600 pmol/l) on collagen production and on α-smooth muscle actin (α-SMA) protein expression was found after 1, 2 and 4 h of incubation. After 24 and 48 h, TGF-β1 stimulated collagen production and α-SMA protein expression in cardiac fibroblasts. Intracellular ROS were not affected by TGF-β1 after 0.5 and 1 h of incubation, began to rise after 2 h, and reached a maximal increase after 4 h. The TGF-β1-stimulated ROS and collagen production is reduced by the ROS inhibitor diphenyleneiodonium chloride (DPI). DPI also decreased the TGF-β1-stimulated α-SMA protein expression in rat cardiac fibroblasts. Our data indicate that the TGF-β1-induced increase in ROS preceded the rise in collagen production and α-SMA protein expression and that ROS inhibition diminished the conversion of fibroblasts into myofibroblasts.

Key words: Alpha-smooth muscle actin; Cardiac fibroblasts; Collagen; Transforming growth factor-beta1


 
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This Article Cited By the following articles

Suppression of TGF-β1/Smad Signaling Pathway by Sesamin Contributes to the Attenuation of Myocardial Fibrosis in Spontaneously Hypertensive Rats
PLOS ONE 2015; 10(3): e0121312.

1
 

How to Cite this Article
Pubmed Style

Purnomo Y, Piccart Y, Coenen T, Pelt Jv, Prihadi JS, Lijnen PJ. Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxid Antioxid Med Sci. 2013; 2(1): 5-10. doi:10.5455/oams.210113.or.025


Web Style

Purnomo Y, Piccart Y, Coenen T, Pelt Jv, Prihadi JS, Lijnen PJ. Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. http://www.ejmoams.com/?mno=31107 [Access: August 20, 2018]. doi:10.5455/oams.210113.or.025


AMA (American Medical Association) Style

Purnomo Y, Piccart Y, Coenen T, Pelt Jv, Prihadi JS, Lijnen PJ. Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxid Antioxid Med Sci. 2013; 2(1): 5-10. doi:10.5455/oams.210113.or.025



Vancouver/ICMJE Style

Purnomo Y, Piccart Y, Coenen T, Pelt Jv, Prihadi JS, Lijnen PJ. Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxid Antioxid Med Sci. (2013), [cited August 20, 2018]; 2(1): 5-10. doi:10.5455/oams.210113.or.025



Harvard Style

Purnomo, Y., Piccart, Y., Coenen, T., Pelt, J. v., Prihadi, J. S. & Lijnen, P. J. (2013) Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxid Antioxid Med Sci, 2 (1), 5-10. doi:10.5455/oams.210113.or.025



Turabian Style

Purnomo, Yudi, Yvette Piccart, Tamara Coenen, Jos van Pelt, John S. Prihadi, and Paul J. Lijnen. 2013. Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxidants and Antioxidants in Medical Science, 2 (1), 5-10. doi:10.5455/oams.210113.or.025



Chicago Style

Purnomo, Yudi, Yvette Piccart, Tamara Coenen, Jos van Pelt, John S. Prihadi, and Paul J. Lijnen. "Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts." Oxidants and Antioxidants in Medical Science 2 (2013), 5-10. doi:10.5455/oams.210113.or.025



MLA (The Modern Language Association) Style

Purnomo, Yudi, Yvette Piccart, Tamara Coenen, Jos van Pelt, John S. Prihadi, and Paul J. Lijnen. "Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts." Oxidants and Antioxidants in Medical Science 2.1 (2013), 5-10. Print. doi:10.5455/oams.210113.or.025



APA (American Psychological Association) Style

Purnomo, Y., Piccart, Y., Coenen, T., Pelt, J. v., Prihadi, J. S. & Lijnen, P. J. (2013) Role of reactive oxygen species in the transforming growth factor-β1-induced collagen production and differentiation of cardiac fibroblasts into myofibroblasts. Oxidants and Antioxidants in Medical Science, 2 (1), 5-10. doi:10.5455/oams.210113.or.025





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