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In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin

Yaw Duah Boakye, Christian Agyare, Susana Oteng Dapaah.

Cited by (2)

Abstract
Objective: Phyllanthus muellerianus (Kuntze) Exell. of the family Euphorbiaceae, is used as a wound healing agent in Ghana and other West African countries. Oxidative stress has been identified to delay wound healing process and subsequently leads to the development of chronic wounds. Hence, the aim of the present study was to investigate the in vivo and in vitro antioxidant properties of aqueous extract of the aerial parts of P.muellerianus (PLE) and its major constituent, geraniin.
Methods: Wound tissues excised from rats treated with PLE (0.25, 0.5 and 1% w/w) and geraniin (0.1, 0.2 and 0.4 % w/w) incorporated in aqueous creams were assayed for the levels of superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO), ascorbate peroxidase (APx) and malondialdehyde (MDA). 1,1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power, total phenolic content and total antioxidant capacity assays were used to measure in vitro antioxidant activity.
Results: SOD, CAT and APx activity increased significantly in both PLE and geraniin-treated wound tissues compared to the untreated tissues. However, MPO activity decreased significantly in all three PLE and 0.2 and 0.4% w/w geraniin-treated wound tissues compared to the untreated tissues. MDA levels decreased significantly in all of the PLE and geraniin-treated wound tissues compared to the untreated tissues. Ferric reducing antioxidant power (FRAP) was observed to be significantly lower for PLE than geraniin.

Key words: Antioxidant, oxidant, wound healing, Phyllanthus muellerianus, geraniin


 
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How to Cite this Article
Pubmed Style

Boakye YD, Agyare C, Dapaah SO. In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxid Antioxid Med Sci. 2016; 5(2): 70-78. doi:10.5455/oams..290616.or.099


Web Style

Boakye YD, Agyare C, Dapaah SO. In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. http://www.ejmoams.com/?mno=228801 [Access: August 18, 2018]. doi:10.5455/oams..290616.or.099


AMA (American Medical Association) Style

Boakye YD, Agyare C, Dapaah SO. In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxid Antioxid Med Sci. 2016; 5(2): 70-78. doi:10.5455/oams..290616.or.099



Vancouver/ICMJE Style

Boakye YD, Agyare C, Dapaah SO. In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxid Antioxid Med Sci. (2016), [cited August 18, 2018]; 5(2): 70-78. doi:10.5455/oams..290616.or.099



Harvard Style

Boakye, Y. D., Agyare, C. & Dapaah, S. O. (2016) In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxid Antioxid Med Sci, 5 (2), 70-78. doi:10.5455/oams..290616.or.099



Turabian Style

Boakye, Yaw Duah, Christian Agyare, and Susana Oteng Dapaah. 2016. In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxidants and Antioxidants in Medical Science, 5 (2), 70-78. doi:10.5455/oams..290616.or.099



Chicago Style

Boakye, Yaw Duah, Christian Agyare, and Susana Oteng Dapaah. "In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin." Oxidants and Antioxidants in Medical Science 5 (2016), 70-78. doi:10.5455/oams..290616.or.099



MLA (The Modern Language Association) Style

Boakye, Yaw Duah, Christian Agyare, and Susana Oteng Dapaah. "In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin." Oxidants and Antioxidants in Medical Science 5.2 (2016), 70-78. Print. doi:10.5455/oams..290616.or.099



APA (American Psychological Association) Style

Boakye, Y. D., Agyare, C. & Dapaah, S. O. (2016) In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin. Oxidants and Antioxidants in Medical Science, 5 (2), 70-78. doi:10.5455/oams..290616.or.099





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