ISSN 2146-8389
 

Original Research (Original Article) 


Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage

Martina Mapelli, Rossella Calo, Laura Marabini.

Abstract
Objective: The aims of our study were to characterize ultraviolet (UV)A- and UVB-induced damages in a keratinocytes cell line (HaCaT), and to evaluate the protective capacities two plant-derivative compounds, namely Thymus vulgaris L leaf extract and thymol, its major component. A polyphenol rich diet has gained wide attention and it is now considered to be a protective agent for human skin, which can be over-exposed to environmental factors and in particular UV light.
Methods: Cells were pretreated for 1 h, in serum-free medium, with thymol (1 μg/ml) or Thymus vulgaris L (1.82 μg/ml) then exposed to different UVA (8-24 J/cm2) or UVB doses (0.016-0.72 J/cm2). Immediately after the UV exposure the intracellular redox status was evaluated by reactive oxygen species quantification and apoptotic events. Genotoxic aspects were evaluated 24 h after the end of irradiations using the alkaline comet assay and the immunostaining of phosphorylated H2AX histone protein (detected 1 h after the end of UV exposure).
Results: The pre-treatment of our experimental model with the two substances confirmed an antioxidant action and anti-apoptotic effect by reducing the cells percentage (sub-G1 phase). Furthermore, thymol and extract of Thymus vulgaris L were able to reduce genotoxic damage. The alkaline comet assay showed that the two substances were capable to decrease DNA damage. Also in this case, Thymus vulgaris L extract is more effective than thymol in decreasing genotoxicity markers.
Conclusions: Our results confirmed the more oxidant UVA and more genotoxic UVB effects. Regarding the protective effect of thymol and Thymus vulgaris L extract, data obtained proved their antioxidant and free-radical scavenging ability as known for phenolic (which our compounds belong to) and polyphenolic compounds. Thymol and mainly Thymus vulgaris L extract were also able to reduce the direct genotoxic damage.

Key words: Genotoxicity; phenolic compounds; ultraviolet radiations; thmol; Thymus vulgaris


 
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REFERENCES
1. Ly C, Yockell-Lelievre J, Ferraro ZM, Arnason JT, Ferrier J, Gruslin A. The effects of dietary polyphenols on reproductive health and early development. Hum Reprod Update 2011; 21:228-48. [DOI via Crossref]    [Pubmed]   
2. Manach C, Scalbert A, Morand A, Remesy C, Jimenez L. Polyphenols: Food sources and bioavailability. Am J Clin Nutr 2004; 79:727-47.
3. Fernandez-Garcia E. Skin protection against UV light by dietary antioxidants. Food Funct 2014; 5:1994-2003. [DOI via Crossref]    [Pubmed]   
4. Sandoval-Acuna C, Ferreira J, Speisky H. Polyphenols and mitochondria: an update on their increasingly emerging ROSscavenging independent actions. Arch Biochem Biophys 2014; 559:75-90. [DOI via Crossref]    [Pubmed]   
5. Afaq F, Katiyar SK. Polyphenols: skin photoprotection and inhibition of photocarcinogenesis. Mini Rev Med Chem 2011; 11:1200-15. [DOI via Crossref]   
6. Afaq F. Natural agents: cellular and molecular mechanisms of photoprotection. Arch Biochem Biophys 2011; 508:144-51. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
7. Chang HP, Sheen LY, Lei YP. The protective role of carotenoids and polyphenols in patients with head and neck cancer. J Chin Med Assoc 2015; 78:89-95. [DOI via Crossref]    [Pubmed]   
8. Sklar LR, Almutawa F, Lim HW, Hamzavi I. Effects of ultraviolet radiation, visible light, and infrared radiation on erythema and pigmentation: a review. Photochem Photobiol Sci 2013; 12:54- 64. [DOI via Crossref]    [Pubmed]   
9. Svobodova A, Vostalova J. Solar radiation induced skin damage: review of protective and preventive options. Int J Radiat Biol 2010; 86:999-1030. [DOI via Crossref]    [Pubmed]   
10. Battie C, Verschoore M. Cutaneous solar ultraviolet exposure and clinical aspects of photodamage. Indian J Dermatol Venereol Leprol 2012; 78:S9-14.
11. Marrot L, Meunier JR. Skin DNA photodamage and its biological consequences. J Am Acad Dermatol 2008; 58:S139-48.
12. Nichols JA, Katiyar SA. Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res 2010; 30:71-83.
13. Cadet J, Douki T. Oxidatively generated damage to DNA by UVA radiation in cells and human skin. J Invest Dermatol 2011; 131:1005-7. [DOI via Crossref]    [Pubmed]   
14. Greinert R, Volkmer B, Henning S, Breitbart EW, Greulich KO, Cardoso MC, Rapp A. UVA-induced DNA double-strand breaks result from the repair of clustered oxidative DNA damages Nucleic Acids Res 2012: 40:10263-73.
15. Cadet J, Mouret S, Ravanat JL, Douki T. Photoinduced damage to cellular DNA: Direct and photosensitized reaction. Photochem Photobiol 2012; 88:1048-65. [DOI via Crossref]    [Pubmed]   
16. Caputo F, Vegliante R, Ghibelli L. Redox modulation of the DNA damage response. Biochem Pharmacol 2012; 84:1292-306. [DOI via Crossref]    [Pubmed]   
17. Runger TM, Kappes UP. Mechanisms of mutation formation with long-wave ultraviolet light (UVA). Photodermatol Photoimmunol Photomed 2008; 24:2-10. [DOI via Crossref]    [Pubmed]   
18. Svobodova A,Walterova D, Vostalova J. Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2006; 150:25-38. [DOI via Crossref]    [Pubmed]   
19. Ikehata K, Ono T. The mechanisms of UV mutagenesis. J Radiat Res 2011; 52:115-25:
20. Cadet J, Grand A, Douki T. Solar UV radiation-induced DNA bipyrimidine photoproducts: formation and mechanistic insights. Top Curr Chem 2014; 356:249-75. [DOI via Crossref]    [Pubmed]   
21. Salucci S, Burattini S, Curzi D, Buontempo F, Martelli AM, Zappia G, Falcieri E, Battistelli M. Antioxidants in the prevention of UVBinduced keratynocyte apoptosis J Photochem Photobiol B 2014; 141:1-9.
22. Peres PS, Terra VA, Guarnier FA, Cecchini R, Cecchini AL. Photoaging and chronological aging profile: understanding oxidation of the skin. J Photochem Photobiol B 2011; 103:93-7. [DOI via Crossref]    [Pubmed]   
23. Lee TH, Do MH, Oh HL, Cho DW, Kim SH, Kim SY. Dietaryfermented soybean suppresses UVB-induced skin inflammation in hairless mice via regulation of the MAPK signaling pathway. J Agric Food Chem 2014; 62:8962-72. [DOI via Crossref]    [Pubmed]   
24. Jang J, Ye BR, Heo SJ, Oh C, Kang DH, Kim JH, Affan A, Yoon KT, Choi YU, Park SC, Han S, Qian ZJ, Jung WK, Choi IW. Photooxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes. Environ Toxicol Pharmacol 2012; 34:926-34. [DOI via Crossref]    [Pubmed]   
25. Hussein MR. Ultraviolet radiation and skin cancer: molecular mechanisms. J Cutan Pathol 2005; 32:191-205. [DOI via Crossref]    [Pubmed]   
26. Junkins-Hopkins JM. Antioxidants and their chemopreventive properties in dermatology. J Am Acad Dermatol 2010; 62:663-5. [DOI via Crossref]    [Pubmed]   
27. Dijk M, Typas D, Mullenders L, Pines A. Insight in the multilevel regulation of NER. Experimental cell research 2014; 329:116-23. [DOI via Crossref]    [Pubmed]   
28. Bergink S, Toussaint W, Luijsterburg MS, Dinant C, Alekseev S, Hoeijmakers JHJ, Dantuma NP, Houtsmuller AB, Vermeulen W.Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23 complex. J Cell Biol 2012; 196:681-8. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
29. Edmonds MJ, Parsons JL. Regulation of base excision repair proteins by ubiquitylation. Experimental cell research 2014; 329:132-8. [DOI via Crossref]    [Pubmed]   
30. Grosso C, Figueiredo AC, Burillo J, Mainar AM, Urieta JS, Barroso JB, Coelho JA, Palavra AM. Composition and antioxidant activity of Thymus vulgaris volatiles: comparison between supercritical fluid extraction and hydrodistillation. J Sep Sci 2010; 33:2211-8. [DOI via Crossref]    [Pubmed]   
31. Undeger U, Basaran A, Degen JH, Basaran N. Antioxidant activities of major thyme ingredients and lack of (oxidative) DNA damage in V79 Chinese hamster lung fibroblast cells at low levels of carvacrol and thymol. Food Chem Toxicol 2009; 47:2037-43. [DOI via Crossref]    [Pubmed]   
32. Kim YS, Hwang JW, Kang SH, Kim EH, Jeon JH, Kim HR, Moon SH, Jeon BT, Park PJ. Thymol from Thymus quinquecostatus Celak. protects against tert-butyl hydroperoxide-induced oxidative stress in Chang cells. J Nat Med 2014; 68:154-62. [DOI via Crossref]    [Pubmed]   
33. Horvathova E, Navarova J, Galova E, Sevcovicova A, Chodakova L, Snahnicanova L, Melusova M, Kozics K, Slamenova D. Assessment of antioxidative, chelating, and DNA-protective effects of selected essential oil components (eugenol, carvacrol, thymol, borneol, eucalyptol) of plants and intact Rosmarinus officinalis oil. J Agric Food Chem 2014; 62:6632-9 [DOI via Crossref]    [Pubmed]   
34. Hashemipour H, Kermanshahi H, Golian A, Veldkamp T. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poult Sci 2013; 92:2059-69 [DOI via Crossref]    [Pubmed]   
35. Archana PR, Nageshwar Rao B, Ballal M, Satish Rao BS. Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity. Mutat Res 2009; 68:70-7. [DOI via Crossref]    [Pubmed]   
36. Ocana A, Reglero G. Effects of thyme extract oils (from Thymus vulgaris, Thymus zygis, and Thymus hyemalis) on cytokine production and gene expression of oxLDL-stimulated THP-1- macrophages. J Obes 2012; 2012:104706. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
37. Azirak S, Rencuzogullari E.The in vivo genotoxic effects of carvacrol and thymol in rat bone marrow cells 2008; Environ Toxicol 23:728-35.
38. Buyukleyla M, Rencuzogullari E.The effects of thymol on sister chromatid exchange, chromosome aberration and micronucleus in human lymphocytes. Ecotoxicol Environ Saf 2009; 72:943-7. [DOI via Crossref]    [Pubmed]   
39. Satooka H, Kubo I. Effects of thymol on B16-F10 melanoma cells. J Agric Food Chem 2012; 60:2746-52. [DOI via Crossref]    [Pubmed]   
40. Stammati A, Bonsi P, Zucco F, Moezelaar R, Alakomi HL, von Wright A. Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Food Chem Toxicol 1999; 37:813-23. [DOI via Crossref]   
41. Shettigar NB, Das S, Rao NB, Rao SBS. Thymol, a monoterpene phenolic derivative of cymene, abrogates mercury-induced oxidative stress resultant cytotoxicity and genotoxicity in hepatocarcinoma cells. Environ Toxicol 2015; 30:968-80. [DOI via Crossref]    [Pubmed]   
42. Nogueira DR, Mitjans M, Infante MR, Vinardell MP. Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications. Int J Pharm 2011; 420:51- 8. [DOI via Crossref]    [Pubmed]   
43. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991; 139:271-9. [DOI via Crossref]   
44. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007; 35:495-516. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
45. WangH, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 1999; 27:612-6.
46. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265-75.
47. Marabini L, Calo R, Fucile S. Genotoxic effects of polychlorinated biphenyls (PCB 153, 138, 101, 118) in a fish cell line (RTG-2). Toxicol In Vitro 2011; 25:1045-52. [DOI via Crossref]    [Pubmed]   
48. Buschini A, Carboni P, Frigerio S, Furlini M, Marabini L, Monarca S, Poli P, Radice S, Rossi C. Genotoxicity and cytotoxicity assessment in lake drinking water produced in a treatment plant. Mutagenesis 2004; 19:341-7. [DOI via Crossref]    [Pubmed]   
49. Azqueta A, Collins AR. The essential comet assay: a comprehensive guide to measuring DNA damage and repair. Arch Toxicol 2013; 87:949-68. [DOI via Crossref]    [Pubmed]   
50. Rapp A, Greulich KA. After double-strand break induction by UVA, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available. J Cell Sci 2004; 117:4935-45. [DOI via Crossref]    [Pubmed]   
51. Ivashkevich A, Redon CE, Nakamura AJ, Martin RF, MartinOA. Use of the gamma-H2AX assay to monitor DNA damage and repair in translational cancer research. Cancer Lett 2012; 327:123-33. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
52. Mah LJ, El-Osta A, Karagiannis TC. GammaH2AX: a sensitive molecular marker of DNA damage and repair. Leukemia 2010; 24:679-86. [DOI via Crossref]    [Pubmed]   
53. Tanaka ZDT, Halicka HD, Traganos F, Seiter K. Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: relation to cell cycle phase. Cell Cycle 2007; 6:371-6. [DOI via Crossref]    [Pubmed]   
54. Wischermann K, Popp S, Moshir S, Scharfetter-Kochanek K, Wlaschek K, de Gruijl F, Hartschuh W, Greinert R,Volkmer B, Faust A, Rapp A, Schmezer P, Boukamp P. UVA radiation causes DNA strand breaks, chromosomal aberrations and tumorigenic transformation in HaCaT skin keratinocytes. Oncogene 2008; 27:4269-80. [DOI via Crossref]    [Pubmed]   
55. Moehrle M. Outdoor sports and skin cancer. Clin Dermatol 2008; 26:12-5. [DOI via Crossref]    [Pubmed]   
56. Wang F, Ge T, Gao Q, Hu L, Yu J, Liu Y. The distribution of biologically effective UV spectral irradiances received on a manikin face that cause erythema and skin cancer. J Photochem Photobiol B 2014; 140:205-14. [DOI via Crossref]    [Pubmed]   
57. Fusenig NE, Boukamp P. Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes. Mol Carcinog 1998; 23:144-58. [DOI via Crossref]   
58. He YY, Pi J, Huang JL, Diwan BA,Waalkes MP, Chignell CF. Chronic UVA irradiation of human HaCaT keratinocytes induces malignant transformation associated with acquired apoptotic resistance. Oncogene 2006; 25:3680-8. [DOI via Crossref]    [Pubmed]   
59. Sander CS, Chang H, Hamm F, Elsner P, Thiele JJ. Role of oxidative stress and the antioxidant network in cutaneous carcinogenesis. Int J Dermatol 2004; 43:326-35. [DOI via Crossref]    [Pubmed]   
60. Calo R, Visone CM, Marabini L. Thymol and Thymus vulgaris L. activity against UVA- and UVB-induced damage in NCTC 2544 cell line. Mutat Res Genet Toxicol Environ Mutagen 2015; 791:30-7. [DOI via Crossref]    [Pubmed]   
61. Serini S, Donato V, Piccioni E, Trombino S, Monego G, Toesca A, Innocenti I, Missori M, De Spirito M, Celleno L, Fasano E, Ranelletti FO, Calviello G. Docosahexaenoic acid reverts resistance to UV-induced apoptosis in human keratinocytes: involvement of COX-2 and HuR. J Nutr Biochem 2011; 22:874-85. [DOI via Crossref]    [Pubmed]   
62. Dinkova-Kostova AT. Phytochemicals as protectors against ultraviolet radiation: versatility of effects and mechanisms. Planta Med 2008; 74:1548-59. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Mapelli M, Calo R, Marabini L. Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxid Antioxid Med Sci. 2016; 5(2): 39-48. doi:10.5455/oams.170416.or.095


Web Style

Mapelli M, Calo R, Marabini L. Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. http://www.ejmoams.com/?mno=222672 [Access: May 22, 2018]. doi:10.5455/oams.170416.or.095


AMA (American Medical Association) Style

Mapelli M, Calo R, Marabini L. Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxid Antioxid Med Sci. 2016; 5(2): 39-48. doi:10.5455/oams.170416.or.095



Vancouver/ICMJE Style

Mapelli M, Calo R, Marabini L. Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxid Antioxid Med Sci. (2016), [cited May 22, 2018]; 5(2): 39-48. doi:10.5455/oams.170416.or.095



Harvard Style

Mapelli, M., Calo, R. & Marabini, L. (2016) Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxid Antioxid Med Sci, 5 (2), 39-48. doi:10.5455/oams.170416.or.095



Turabian Style

Mapelli, Martina, Rossella Calo, and Laura Marabini. 2016. Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxidants and Antioxidants in Medical Science, 5 (2), 39-48. doi:10.5455/oams.170416.or.095



Chicago Style

Mapelli, Martina, Rossella Calo, and Laura Marabini. "Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage." Oxidants and Antioxidants in Medical Science 5 (2016), 39-48. doi:10.5455/oams.170416.or.095



MLA (The Modern Language Association) Style

Mapelli, Martina, Rossella Calo, and Laura Marabini. "Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage." Oxidants and Antioxidants in Medical Science 5.2 (2016), 39-48. Print. doi:10.5455/oams.170416.or.095



APA (American Psychological Association) Style

Mapelli, M., Calo, R. & Marabini, L. (2016) Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage. Oxidants and Antioxidants in Medical Science, 5 (2), 39-48. doi:10.5455/oams.170416.or.095





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