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Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an <i>in vitro</i> study

Amal Ajaweed Sulaiman, Saad Abdulrahman Hussain.

Cited by (1)

Abstract
The non-enzymatic, free radical-mediated peroxidation of erythrocyte membranes is now correlated with a variety of pathological conditions, and considered as a primary event associated with hematotoxicity of variety of drugs and chemicals that induce erythrocyte hemolysis. The plant derived polyphenols including anthocyanins have been suggested to enhance red blood cell resistance to oxidative stress both <i>in vitro</i> and <i>in vivo</i>. The present study was designed to investigate the concentration and time dependent anti-hemolytic activity of anthocyanins using <i>in vitro</i> models of azo compounds-induced erythrocyte membrane peroxidative damage. Three different concentrations (1, 0.1 and 0.01 mg/ml) of anthocyanins solution were incubated with erythrocytes suspension 30 min before addition of either water soluble (AAPH) or lipid soluble (AMVN) free radical initiators. The level of hemoglobin released into the solution was determined spectrophotometrically, both in presence and absence of anthocyanins. Moreover, the most effective concentration was selected to be added at different time intervals (zero time, 2 and 3 hours) post challenge with either AAPH or AMVN and the extent of hemolysis was determined as mentioned before. The results showed that anthocyanins, concentration dependently attenuated hemolysis induced by azo compounds, and substantial cytoprotective effects of anthocyanins were observed when they added simultaneously with azo compounds, while delayed addition of anthocyanins for 2 and 3 h diminished but not abolished the protective effect of anthocyanins. In conclusion, anthocyanins can effectively display concentration and time dependent cytoprotective properties against azo compounds-induced peroxidative hemolytic damage.

Key words: Anthocyanins; Cytoprotection; Oxidative challenge


 
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This Article Cited By the following articles

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Journal of Functional Foods 2018; 43(): 224.

1
 

How to Cite this Article
Pubmed Style

Sulaiman AA, Hussain SA. Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxid Antioxid Med Sci. 2012; 1(2): 133-140. doi:10.5455/oams.250712.or.010


Web Style

Sulaiman AA, Hussain SA. Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. http://www.ejmoams.com/?mno=20958 [Access: May 22, 2018]. doi:10.5455/oams.250712.or.010


AMA (American Medical Association) Style

Sulaiman AA, Hussain SA. Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxid Antioxid Med Sci. 2012; 1(2): 133-140. doi:10.5455/oams.250712.or.010



Vancouver/ICMJE Style

Sulaiman AA, Hussain SA. Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxid Antioxid Med Sci. (2012), [cited May 22, 2018]; 1(2): 133-140. doi:10.5455/oams.250712.or.010



Harvard Style

Sulaiman, A. A. & Hussain, S. A. (2012) Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxid Antioxid Med Sci, 1 (2), 133-140. doi:10.5455/oams.250712.or.010



Turabian Style

Sulaiman, Amal Ajaweed, and Saad Abdulrahman Hussain. 2012. Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxidants and Antioxidants in Medical Science, 1 (2), 133-140. doi:10.5455/oams.250712.or.010



Chicago Style

Sulaiman, Amal Ajaweed, and Saad Abdulrahman Hussain. "Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study." Oxidants and Antioxidants in Medical Science 1 (2012), 133-140. doi:10.5455/oams.250712.or.010



MLA (The Modern Language Association) Style

Sulaiman, Amal Ajaweed, and Saad Abdulrahman Hussain. "Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study." Oxidants and Antioxidants in Medical Science 1.2 (2012), 133-140. Print. doi:10.5455/oams.250712.or.010



APA (American Psychological Association) Style

Sulaiman, A. A. & Hussain, S. A. (2012) Concentration and time dependent cytoprotective effects of anthocyanins against oxidative hemolysis induced by water and lipid soluble free radical initiators: an in vitro study. Oxidants and Antioxidants in Medical Science, 1 (2), 133-140. doi:10.5455/oams.250712.or.010





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