ISSN 2146-8389
 

Original Research (Original Article) 


The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica

Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano.

Cited by (1)

Abstract
Objective: Medihoneyģ is considered up to date the only antioxidant therapy specifically approved by the United States Food and Drug Administration (FDA) for wound healing. This is the first study that evaluates the antioxidant capacity and immunomodulatory activity of stingless bee honeys in comparison to Medihoneyģ. The goal of this study was to evaluate in vitro the potentials of Meliponini honey from Costa Rica as an antioxidant wound dressing agent.
Materials and Methods: A total of fifty-seven honeys belonging to the Tetragonisca angustula (n = 36) and Melipona beecheii (n = 21) species were studied. Following tests were carried out: screening of radical scavenging activity by the 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay, superoxide anion radical scavenging assay, inhibition of xanthine oxidase (XO), total phenolic content by the Folin-Ciocalteu reagent, and the oxygen radical absorbance capacity (ORAC) assay. Furthermore, the effect of honey over reactive oxygen species (ROS) produced by human polymorphonuclear leucocytes (PMNL) was evaluated using luminol and a Cypridina luciferin analog as luminescence probes.
Results: The positive correlation between the total phenolic content and the ORAC value reported herein, suggests that the antioxidant capacities of the stingless bee honeys examined are given in a major grade to their phenolic constituents. According to present results, the in vitro immunomodulatory activities are explained mainly due to the radical scavenging effect and to the XO inhibition. Both mechanisms may play a role in the impairment of the inflammatory process and the promotion of redox homeostasis.
Conclusion: The T.angustula honeys showed antioxidant capacities not statistically different to Medihoneyģ. In addition, their radical scavenging activity over ROS produced by human PMNLs and inhibition of XO justify their ethnopharmacological use as a wound dressing and further research towards novel wound-healing developments.

Key words: Honey, immunomodulatory, Medihoney, Meliponini


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Gabriel Zamora
Articles by Kees Beukelman
Articles by Bert van den Berg
Articles by Maria Laura Arias
Articles by Eduardo Umana
Articles by Ingrid Aguilar
Articles by Linda Quarles van Ufford
Articles by Edwin van den Worm
Articles by Natalia Fallas
Articles by Rebeca Solorzano
on Google
on Google Scholar
Article Statistics
 Viewed: 3577
Downloaded: 2476
Cited: 1

REFERENCES
1. Jones R. Honey and healing through ages. In: Munn P, Jones R, editors. Honey and Healing. Cardiff: International Bee Research Association; 2001. p. 1-4.
2. Cooper R. How does honey heal wounds? In: Munn P, Jones R editor. Honey and Healing. Cardiff: International Bee Research Association; 2001. p. 27-34.
3. Cooper R. The modern use of honey in the treatment of wounds. Bee World 2005;86:110-3. [DOI via Crossref]   
4. Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001;14:244-69. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
5. Howell-Jones RS, Wilson MJ, Hill KE, Howard AJ, Price PE, Thomas DW. A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrob Chemother 2005;55:143-9. [DOI via Crossref]    [Pubmed]   
6. Salyers AA, Whitt DD. Revenge of the Microbes: How Bacterial Resistance is Undermining the Antibiotic Miracle. Washington DC, USA: American Society for Microbiology Press; 2005. p. 186. [DOI via Crossref]   
7. Cooper RA, Halas E, Molan PC. The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. J Burn Care Rehabil 2002;23:366-70. [DOI via Crossref]    [Pubmed]   
8. Lusby PE, Coombes A, Wilkinson JM. Honey: A potent agent for wound healing? J Wound Ostomy Continence Nurs 2002;29:295-300. [DOI via Crossref]   
9. Molan PC. The evidence supporting the use of honey as a wound dressing. Int J Low Extrem Wounds 2006;5:40-54. [DOI via Crossref]    [Pubmed]   
10. Simon A, Sofka K, Wiszniewsky G, Blaser G, Bode U, Fleischhack G. Wound care with antibacterial honey (Medihoney) in pediatric hematology-oncology. Support Care Cancer 2006;14:91-7. [DOI via Crossref]    [Pubmed]   
11. Summary for Derma Sciences OTC APIMED, Medihoney Primary and Medihoney 100% Honey Dressings with Active Manuka Honey. In: Federal Drug Administration. Available from: http://www.accessdata.fda.gov/cdrh_docs/pdf8/K081584.pdf. [Last accessed on 2015 Mar 03].
12. Fitzmaurice SD, Sivamani RK, Isseroff RR. Antioxidant therapies for wound healing: A clinical guide to currently commercially available products. Skin Pharmacol Physiol 2 011;24:113-26.
13. Michener CD. The Bees of the World. Maryland, USA: Johns Hopkins University Press; 2000. p. 992.
14. Kent RB. Mesoamerican stingless bees. J Cult Geogr 1984;4:14-28. [DOI via Crossref]   
15. de Jong H. The land of corn and honey. The keeping of stingless bees (meliponiculture) in the ethno-ecological environment of Yucat√°n Mexico and El Salvador. Utrecht, The Netherlands: Dissertation, Utrecht University; 1999.
16. Cortopassi-Laurino M, Imperatriz-Fonseca VL, Roubik DW, Dollin A, Heard T, Aguilar I, et al. Global meliponiculture: Challenges and opportunities. Apidologie 2006;37:275-92. [DOI via Crossref]   
17. Vit P, Medina M, Enr√≠quez ME. Quality standards for the medicinal use of Meliponinae honey in Guatemala, Mexico and Venezuela. Bee World 2004;85:2-5. [DOI via Crossref]   
18. Dardón MJ, Enríquez E. Caracterización fisicoquímica y antimicrobiana de la miel de nueve especies de abejas sin aguijón (Meliponini) de Guatemala. Interciencia 2008; 33(12):916-922.
19. Aguilar I, Herrera E, Zamora LG. Stingless bees of Costa Rica. In: Vit P, Pedro SR, Roubik D, editors. Pot Honey: A Legacy of Stingless Bees. New York: Springer; 2013. p. 113-24. [DOI via Crossref]   
20. Zamora LG, Beukelman CJ, van den Berg AJ, Arias ML, Uma-a E, Aguilar I, et al. The antimicrobial activity of stingless bee honeys proceeding from Costa Rica. J Apic Res 2014;53:503-13. [DOI via Crossref]   
21. Ancerewicz J, Migliavacca E, Carrupt PA, Testa B, Br√©e F, Zini R, et al. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radic Biol Med 1998;25:113-20. [DOI via Crossref]   
22. Beukelman CJ, van den Berg AJ, Hoekstra MJ, Uhl R, Reimer K, Mueller S. Anti-inflammatory properties of a liposomal hydrogel with povidone-iodine (Repithel) for wound healing in vitro. Burns 2008;34:845-55. [DOI via Crossref]    [Pubmed]   
23. Singleton VL, Orthofer R, Lamuela-Raventos, RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152-78. [DOI via Crossref]   
24. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56. [DOI via Crossref]    [Pubmed]   
25. Zamora LG, Arias ML, Aguilar I, Uma-a E. Costa Rican Pot Honey: Its medicinal use and antibacterial effect. In: Vit P, Pedro SR, Roubik D, editors. Pot Honey: A Legacy of Stingless Bees. New York: Springer; 2013. p. 507-12. [DOI via Crossref]   
26. Gillissen A, Bartling A, Schoen S, Schultze-Werninghaus G. Antioxidant function of ambroxol in mononuclear and polymorphonuclear cells in vitro. Lung 1997;175:235-42. [DOI via Crossref]    [Pubmed]   
27. Halkes SBA. Filipendula ulmaria: A Study on the Immunomodulatory Activity of Extracts and Constituents. Utrecht, The Netherlands: Dissertation, Utrecht University; 1998.
28. K√∂ller M, Clasbrummel B, Kollig E, Hahn MP, Muhr G. Major injury induces increased production of interleukin-10 in human granulocyte fractions. Langenbecks Arch Surg 1998;383:460-5. [DOI via Crossref]    [Pubmed]   
29. Labro MT. Interference of antibacterial agents with phagocyte functions: Immunomodulation or "immuno-fairy tales"? Clin Microbiol Rev 2000;13:615-50. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
30. Simons JM, Hart BA, Ip Vai Ching TR, Van Dijk H, Labadie RP. Metabolic activation of natural phenols into selective oxidative burst agonists by activated human neutrophils. Free Radic Biol Med 1990;8:251-8. [DOI via Crossref]   
31. Hofer HP, Kukovetz E, Petek W, Schweighofer F, Wildburger R, Schaur RJ. Released PMN elastase: An indicator of postsurgical uneventful wound healing and early inflammatory complications. A contribution to the search for objective criteria in wound healing monitoring. Injury 1995;26:103-6. [DOI via Crossref]   
32. Yasuda M, Shimizu S, Tokuyama S, Watanabe T, Kiuchi Y, Yamamoto T. A novel effect of polymorphonuclear leukocytes in the facilitation of angiogenesis. Life Sci 2000;66:2113-21. [DOI via Crossref]   
33. Braga PC, Dal Sasso M, Fonti E, Culici M. Antioxidant activity of bisabolol: Inhibitory effects on chemiluminescence of human neutrophil bursts and cell-free systems. Pharmacology 2009;83:110-5. [DOI via Crossref]    [Pubmed]   
34. Fialkow L, Wang Y, Downey GP. Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function. Free Radic Biol Med 2007;42:153-64. [DOI via Crossref]    [Pubmed]   
35. Huo Y, Qiu WY, Pan Q, Yao YF, Xing K, Lou MF. Reactive oxygen species (ROS) are essential mediators in epidermal growth factor (EGF)- stimulated corneal epithelial cell proliferation, adhesion, migration, and wound healing. Exp Eye Res 2009;89:876-86. [DOI via Crossref]    [Pubmed]   
36. Miller RA, Britigan BE. Role of oxidants in microbial pathophysiology. Clin Microbiol Rev 1997;10:1-18. [Pubmed]    [PMC Free Fulltext]   
37. Sch√∂nfelder U, Abel M, Wiegand C, Klemm D, Elsner P, Hipler UC. Influence of selected wound dressings on PMN elastase in chronic wound fluid and their antioxidative potential in vitro. Biomaterials 2005;26:6664-73. [DOI via Crossref]    [Pubmed]   
38. Azad N, Rojanasakul Y, Vallyathan V. Inflammation and lung cancer: Roles of reactive oxygen/nitrogen species. J Toxicol Environ Health B Crit Rev 2008;11:1-15. [DOI via Crossref]    [Pubmed]   
39. van den Worm E. Investigations on Apocynin, a Potent NADPH Oxidase Inhibitor. Utrecht, The Netherlands: Dissertation, Utrecht University; 2001.
40. Braga PC, Dal Sasso M, Culici M, Spallino A, Falchi M, Bertelli A, et al. Antioxidant activity of Calendula officinalis extract: Inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopy. Pharmacology 2009;83:348-55. [DOI via Crossref]    [Pubmed]   
41. Link EM, Riley PA. Role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system. Biochem J 1988;249:391-9. [Pubmed]    [PMC Free Fulltext]   
42. Yamakawa Y, Takano M, Patel M, Tien N, Takada T, Bulkley GB. Interaction of platelet activating factor, reactive oxygen species generated by xanthine oxidase, and leukocytes in the generation of hepatic injury after shock/resuscitation. Ann Surg 2000;231:387-98. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
43. Segal BH, Sakamoto N, Patel M, Maemura K, Klein AS, Holland SM, et al. Xanthine oxidase contributes to host defense against Burkholderia cepacia in the p47(phox-/-) mouse model of chronic granulomatous disease. Infect Immun 2000;68:2374-8. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
44. Kang J, Li Z, Wu T, Jensen GS, Schauss AG, Wu X. Antioxidant capacities of flavonoid compounds isolated from acai pulp (Euterpe oleracea Mart.). Food Chem 2010;122:610-7. [DOI via Crossref]   
45. Koko WS, Mesaik MA, Yousaf S, Galal M, Choudhary MI. In vitro immunomodulating properties of selected Sudanese medicinal plants. J Ethnopharmacol 2008;118:26-34. [DOI via Crossref]    [Pubmed]   
46. Fang FC. Antimicrobial reactive oxygen and nitrogen species: Concepts and controversies. Nat Rev Microbiol 2004;2:820-32. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

An insight into the antibiofilm properties of Costa Rican stingless bee honeys
Journal of Wound Care 2017; 26(4): 168.

1
 

How to Cite this Article
Pubmed Style

Zamora G, Beukelman K, Berg Bvd, Arias ML, Umana E, Aguilar I, Ufford LQv, Worm Evd, Fallas N, Solorzano R. The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxid Antioxid Med Sci. 2015; 4(1): 49-55. doi:10.5455/oams.180415.or.084


Web Style

Zamora G, Beukelman K, Berg Bvd, Arias ML, Umana E, Aguilar I, Ufford LQv, Worm Evd, Fallas N, Solorzano R. The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. http://www.ejmoams.com/?mno=184173 [Access: April 21, 2018]. doi:10.5455/oams.180415.or.084


AMA (American Medical Association) Style

Zamora G, Beukelman K, Berg Bvd, Arias ML, Umana E, Aguilar I, Ufford LQv, Worm Evd, Fallas N, Solorzano R. The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxid Antioxid Med Sci. 2015; 4(1): 49-55. doi:10.5455/oams.180415.or.084



Vancouver/ICMJE Style

Zamora G, Beukelman K, Berg Bvd, Arias ML, Umana E, Aguilar I, Ufford LQv, Worm Evd, Fallas N, Solorzano R. The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxid Antioxid Med Sci. (2015), [cited April 21, 2018]; 4(1): 49-55. doi:10.5455/oams.180415.or.084



Harvard Style

Zamora, G., Beukelman, K., Berg, B. v. d., Arias, M. L., Umana, E., Aguilar, I., Ufford, L. Q. v., Worm, E. v. d., Fallas, N. & Solorzano, R. (2015) The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxid Antioxid Med Sci, 4 (1), 49-55. doi:10.5455/oams.180415.or.084



Turabian Style

Zamora, Gabriel, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, and Rebeca Solorzano. 2015. The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxidants and Antioxidants in Medical Science, 4 (1), 49-55. doi:10.5455/oams.180415.or.084



Chicago Style

Zamora, Gabriel, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, and Rebeca Solorzano. "The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica." Oxidants and Antioxidants in Medical Science 4 (2015), 49-55. doi:10.5455/oams.180415.or.084



MLA (The Modern Language Association) Style

Zamora, Gabriel, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, and Rebeca Solorzano. "The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica." Oxidants and Antioxidants in Medical Science 4.1 (2015), 49-55. Print. doi:10.5455/oams.180415.or.084



APA (American Psychological Association) Style

Zamora, G., Beukelman, K., Berg, B. v. d., Arias, M. L., Umana, E., Aguilar, I., Ufford, L. Q. v., Worm, E. v. d., Fallas, N. & Solorzano, R. (2015) The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica. Oxidants and Antioxidants in Medical Science, 4 (1), 49-55. doi:10.5455/oams.180415.or.084





Most Viewed Articles
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Effects of silymarin, N-acetylcysteine and selenium in the treatment of papulopustular acne
    Haidar Hamid Al-Anbari, Ahmed Salih Sahib, Ahmed R. Abu Raghif
    Oxid Antioxid Med Sci. 2012; 1(3): 201-207
    » Abstract & References » doi: 10.5455/oams.290912.or.019

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043

  • Most Downloaded
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica
    Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano
    Oxid Antioxid Med Sci. 2015; 4(1): 49-55
    » Abstract & References » doi: 10.5455/oams.180415.or.084

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Protective properties of complex of quercetin, selenium, catechins and curcumin against DNA damage
    Jana Kadrabova, Marica Krajcovicova-Kudlackova, Alexander Madaric, Martina Valachovicova, Csilla Mislanova, Maria Korenovska
    Oxid Antioxid Med Sci. 2012; 1(3): 179-184
    » Abstract & References » doi: 10.5455/oams.180912.or.018

  • Airway antioxidant capacity and pH in chronic obstructive pulmonary disease
    Wei Lee, Hsien Loo, Paul S. Thomas
    Oxid Antioxid Med Sci. 2012; 1(3): 153-160
    » Abstract & References » doi: 10.5455/oams.300812.or.016

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001

  • Most Cited Articles
  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009
    Cited : 12 times [Click to see citing articles]

  • Role of free radicals and antioxidants in gynecological cancers: current status and future prospects
    Lokanatha Valluru, Subramanyam Dasari, Rajendra Wudayagiri
    Oxid Antioxid Med Sci. 2014; 3(1): 15-26
    » Abstract & References » doi: 10.5455/oams.201113.rv.011
    Cited : 10 times [Click to see citing articles]

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038
    Cited : 10 times [Click to see citing articles]

  • Silymarin and naringenin protects nicotine induced oxidative stress in young rats
    Anshu Jain, Nidhi Dwivedi, Rakesh Bhargava, Swaran J.S. Flora
    Oxid Antioxid Med Sci. 2012; 1(1): 41-49
    » Abstract & References » doi: 10.5455/oams.130412.or.004
    Cited : 9 times [Click to see citing articles]

  • Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats
    Fatmah Ali Matough, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, Jamaludin Mohamed
    Oxid Antioxid Med Sci. 2012; 1(1): 59-68
    » Abstract & References » doi: 10.5455/oams.300412.or.006
    Cited : 8 times [Click to see citing articles]

  • Peroxidative index as novel marker of hydrogen peroxide involvement in lipid peroxidation from coal dust exposure
    Nia Kania, Bambang Setiawan, Edi Widjajanto, Nurdiana Nurdiana, M. Aris Widodo, H.M.S. Chandra Kusuma
    Oxid Antioxid Med Sci. 2012; 1(3): 209-215
    » Abstract & References » doi: 10.5455/oams.031012.or.020
    Cited : 8 times [Click to see citing articles]

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043
    Cited : 8 times [Click to see citing articles]

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001
    Cited : 6 times [Click to see citing articles]

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005
    Cited : 6 times [Click to see citing articles]

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051
    Cited : 6 times [Click to see citing articles]