Antioxidant role of plumbagin in modification of radiation-induced oxidative damage

Abstract

Ramachandran Gangabhagirathi , Ravi Joshi

Objective: Plumbagin is a well-known ingredient of Plumbago zeylanica, which is used in Ayurveda for its multiple therapeutic actions. We have attempted to understand protective effects of plumbagin against gamma radiation-induced damage to lipids, proteins, and enzymes of rat liver mitochondria in vitro. Methods: Rat liver mitochondrial preparation of female Wistar rats has been exposed to gamma radiation (450 Gy) to induce oxidative damage of its components. The protection offered by increasing concentration of plumbagin during irradiation for lipids, proteins and enzymes of rat liver mitochondrial preparation has been studied. The protection exerted by plumbagin (50-150 µM) has been studied using different standard assays for lipids (thiobarbituric acid reactive substance and ferrous oxidation in xylenol orange), proteins (dinitrophenylhydrazine and dithiobisnitrobenzoic acid), glutathione (fluorescence), superoxide dismutase (epinephrine oxidation) and succinate dehydrogenase (dichlorophenolindophenol reduction). Results: Plumbagin has been found to inhibit lipid peroxidation and protein carbonyl formation; protect thiols (glutathione and protein) and enzyme activity (superoxide dismutase and succinate dehydrogenase) in a concentration dependent manner. Conclusion: The protection provided by plumbagin to lipids, proteins and enzymes of rat liver mitochondrial preparation against gamma radiation-induced damage suggest that it can provide radioprotection in the living systems.

https://tipobette.com https://vdcasinoyagiris.com https://venusbetting.com https://sahabetting.com https://sekabete.com https://sahabete-giris.com https://onwine-giris.com https://matadorbet-giris.com https://casibomkayit.com https://casibomba.com https://casiboms.com https://casinoplusa.org https://casibomlink.com https://yenicasibom.com https://jojobetegiris.com https://jojoguncel.com https://jojobetyeni.com https://girisgrandbetting.com https://pashabetegiris.com https://grandbettingyeni.com