ISSN 2146-8389
 

Original Research (Original Article) 


The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta.

Cited by (4)

Abstract
Objective: Present study reports the modulatory effect of a novel formulation (G-002M), prepared from three isolated molecules of Podophyllum hexandrum rhizomes on radiation induced oxidative stress in lung and liver tissues of strain A mice.
Methods: Mice, administered with G-002M formulation 1 h prior to lethal (9Gy) radiation exposure dose, were sacrificed at different time intervals. Lung and liver tissues were processed to assess the expression of extracellular superoxide dismutase (EC-SOD) by immunohistochemistry and Western blotting techniques. Histological alterations along with the biochemical determinations, using as reported markers of hepatic and lung oxidative stress were recorded. Pre-administration of G-002M resulted in significant restoration of radiation induced decline in the expression of EC-SOD in both lung and liver tissues.
Results: In lungs, radiation mediated histological alterations like alveolar edema, interalveolar septa thickening, inflammation and infiltration of inflammatory cells into alveolar spaces were significantly prevented by G-002M pre-treatment. Histopathological features of liver in response to radiation such as steatosis, deformed cell structure, vacuolization of the cytoplasm and injury of hepatocyte membranes were also minimized by formulation pre-treatment. The formulation could successfully inhibit radiation induced malondialdehyde (MDA) content and counter the radiation induced depletion in SOD, catalase, glutathione reductase, glutathione S-transferase and glutathione, in serum, lung and liver tissues. Radiation induced alterations in serum aspartate aminotransferase, alanine tranaminase, cholesterol, high-density lipoprotein, low-density lipoprotein, triglycerides, alkaline phosphatase, total protein, albumin and lactate dehydrogenase were maintained by G-002M pre-treatment.
Conclusion: These results demonstrated that the bioactive phyto-constituents of Podophyllum hexandrum could significantly protect lung and liver against radiation, predominantly by reduction in lipid peroxidation and elevation of EC-SOD along with a set of endogenous defense enzymes.

Key words: Extracellular superoxide dismutase; Immunohistochemistry; Podophyllum hexandrum; Radiation; Radioprotection; Reactive oxygen species


 
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How to Cite this Article
Pubmed Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxid Antioxid Med Sci. 2014; 3(1): 51-64. doi:10.5455/oams.261213.or.057


Web Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. http://www.ejmoams.com/?mno=43780 [Access: November 16, 2018]. doi:10.5455/oams.261213.or.057


AMA (American Medical Association) Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxid Antioxid Med Sci. 2014; 3(1): 51-64. doi:10.5455/oams.261213.or.057



Vancouver/ICMJE Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxid Antioxid Med Sci. (2014), [cited November 16, 2018]; 3(1): 51-64. doi:10.5455/oams.261213.or.057



Harvard Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta (2014) The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxid Antioxid Med Sci, 3 (1), 51-64. doi:10.5455/oams.261213.or.057



Turabian Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. 2014. The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxidants and Antioxidants in Medical Science, 3 (1), 51-64. doi:10.5455/oams.261213.or.057



Chicago Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. "The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice." Oxidants and Antioxidants in Medical Science 3 (2014), 51-64. doi:10.5455/oams.261213.or.057



MLA (The Modern Language Association) Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta. "The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice." Oxidants and Antioxidants in Medical Science 3.1 (2014), 51-64. Print. doi:10.5455/oams.261213.or.057



APA (American Psychological Association) Style

Savita Verma, Bhargab Kalita, Rashmi Saini, Manju Lata Gupta (2014) The protective effect of Podophyllum hexandrum on hepato-pulmonary toxicity in irradiated mice. Oxidants and Antioxidants in Medical Science, 3 (1), 51-64. doi:10.5455/oams.261213.or.057





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