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Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects

Oladimeji S. Tugbobo, Omotade I. Oloyede, Olusola B. Adewale.

Abstract
The antioxidant potentials of Allium sativum extract was studied in bone marrow cells of albino rats using micronucleus assay with the use of 100 mg/ml crude garlic extract as dietary supplement via oral gavage. The rats were divided into three groups: A, distilled water; B, lead acetate; C, garlic extract + lead acetate. After the short-term exposure, rats were sacrificed by cervical dislocation and chromosomal preparations were made from bone marrow according to colchicines-hypotonic-fixation-air-drying-Giemsa schedule. The cytogenic end points observed were chromosomal aberrations and tissue damage. The chromosomal aberration induced by lead was reduced significantly in animals fed with the extract in group C while lead acetate administered to animals in group B was highly mutagenic. Besides, the antioxidant properties of garlic was further demonstrated in the in vitro experiment where it provided significant protection to thiobarbituric reactive substances inhibition, and reduced and oxidized glutathione contents in liver tissues. This suggests their ability to act as free-radical scavengers and in protecting the cell against oxidative stress for normal cellular functions. The results further harp on the involvement of reactive oxygen species in lead toxicity and also revealed the beneficial role of garlic therapeutic efficacy which indicates the antimutagenic and antioxidant potentials of garlic against oxidative stress and mutation.

Key words: Chromosomal aberration; Garlic; Lead; Oxidative stress


 
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REFERENCES
1.Humphreys DJ. Effects of exposure to excessive quantities of lead on animals. Br Vet J 1991; 147:18-30.

http://dx.doi.org/10.1016/0007-1935(91)90063-S

[Pubmed]   


2.Brautbar MD. Industrial solvents and kidney disease. Int J Occup Environ Health 2004; 10:79-83.

http://dx.doi.org/10.1179/oeh.2004.10.1.79

[
Pubmed]   


3.Ercal N, Treeratphan P, Hammond TC, Matthews RH, Grannemann NH, Spitz DR. In vivo indices of oxidative stress in lead-exposed C57BL/6 mice are reduced by treatment with meso-2,3-dimercaptosuccinic acid or N-acetylcysteine. Free Radic Biol Med 1996; 21:157-61.

http://dx.doi.org/10.1016/0891-5849(96)00020-2

[
Pubmed]   


4.Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721-4.

http://dx.doi.org/10.1016/S0140-6736(94)92211-X

[
Pubmed]   


5.Hilderbrand DC, Der R, Griffin WT, Fahim MS. Effect of lead acetate on reproduction. Am J Obstet Gynecol 1973; 115:1058-65.

[
Pubmed]   


6.Poddar S, Mukherjee P, Talukder G, Sharma A. Dietary protection by iron against clastogenic effects of short-term exposure to arsenic in mice in vivo. Food Chem Toxicol 2000; 38:735-7.

http://dx.doi.org/10.1016/S0278-6915(00)00062-4

[
Pubmed]   


7.Choudhury AR, Das T, Sharma A, Talukder G. Inhibition of clastogenic effects of arsenic through continued oral administration of garlic extract in mice in vivo. Mutat Res 1997; 392:237-42

[
Pubmed]   


8.Mansell P, Reckless JP. Garlic. Br Med J 1991; 303:379-80.

http://dx.doi.org/10.1136/bmj.303.6799.379

[
Pubmed]   


9.Sharma AK, Sharma A. Chromosome Techniques – A Manual. 6th edition, Harwood Academic Publishers, Chur, Switzerland, p 368, 1994.

[
Pubmed]   


10.Alasoadura OH. Cytogenic study in a species of anurans (amphibians) Bufo regularis. Dissertation, Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria, 1996.


11.Al-Shehri AH, Al-Saleh AA. Karyotype of amphibian in Saudi Arabia. 1.The karyotype of Rana ridibunda. J Biol Sci 2005; 5:335-8.

http://dx.doi.org/10.3923/jbs.2005.335.338


12.Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-8.

http://dx.doi.org/10.1016/0003-2697(79)90738-3

[
Pubmed]   


13.Beutler E, Gelbart T. Improved assay of the enzymes of glutathione synthesis: gamma-glutamylcysteine synthetase and glutathione synthetase. Clin Chim Acta 1986; 158:115-23.

http://dx.doi.org/10.1016/0009-8981(86)90122-1

[
Pubmed]   


14.Sokal RR, Rohf FJ. Single classification of analysis of variance. In: Introduction to Biostatistics, WH Freeman & Co, San Francisco, p 363, 1987.


15.Sharma A, Talukder G. Effects of metals on chromosomes of higher organisms. Environ Mutagen 1987; 9:191-226.

http://dx.doi.org/10.1002/em.2860090210

[
Pubmed]   


16.Monteiro HP, Bechara EJ, Abdalla DS. Free radicals involvement in neurological porphyrias and lead poisoning. Mol Cell Biochem 1991; 103:73-83.

http://dx.doi.org/10.1007/BF00229595

[
Pubmed]   


17.Flora SJ, Pande M, Mehta A. Beneficial effect of combined administration of some naturally occurring antioxidant and thiol chelators in lead treatment. Chem Biol Interact 2003; 145:267-80.

http://dx.doi.org/10.1016/S0009-2797(03)00025-5

[
Pubmed]   


18.Tugbobo OS, Oloyede OI, Daramola AO. Protection by garlic extract against lead induced tissue atrophy in albino rats in vivo. Arch Appl Sci Res 2012; 4:65-71.


How to Cite this Article
Pubmed Style

Tugbobo OS, Oloyede OI, Adewale OB. Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxid Antioxid Med Sci. 2014; 3(1): 79-82. doi:10.5455/oams.061213.br.008


Web Style

Tugbobo OS, Oloyede OI, Adewale OB. Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. http://www.ejmoams.com/?mno=40927 [Access: May 22, 2018]. doi:10.5455/oams.061213.br.008


AMA (American Medical Association) Style

Tugbobo OS, Oloyede OI, Adewale OB. Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxid Antioxid Med Sci. 2014; 3(1): 79-82. doi:10.5455/oams.061213.br.008



Vancouver/ICMJE Style

Tugbobo OS, Oloyede OI, Adewale OB. Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxid Antioxid Med Sci. (2014), [cited May 22, 2018]; 3(1): 79-82. doi:10.5455/oams.061213.br.008



Harvard Style

Tugbobo, O. S., Oloyede, O. I. & Adewale, O. B. (2014) Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxid Antioxid Med Sci, 3 (1), 79-82. doi:10.5455/oams.061213.br.008



Turabian Style

Tugbobo, Oladimeji S., Omotade I. Oloyede, and Olusola B. Adewale. 2014. Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxidants and Antioxidants in Medical Science, 3 (1), 79-82. doi:10.5455/oams.061213.br.008



Chicago Style

Tugbobo, Oladimeji S., Omotade I. Oloyede, and Olusola B. Adewale. "Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects." Oxidants and Antioxidants in Medical Science 3 (2014), 79-82. doi:10.5455/oams.061213.br.008



MLA (The Modern Language Association) Style

Tugbobo, Oladimeji S., Omotade I. Oloyede, and Olusola B. Adewale. "Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects." Oxidants and Antioxidants in Medical Science 3.1 (2014), 79-82. Print. doi:10.5455/oams.061213.br.008



APA (American Psychological Association) Style

Tugbobo, O. S., Oloyede, O. I. & Adewale, O. B. (2014) Dietary protection by garlic extract against lead induced oxidative stress and genetic birth defects. Oxidants and Antioxidants in Medical Science, 3 (1), 79-82. doi:10.5455/oams.061213.br.008





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