ISSN 2146-8389
 

Original Research (Original Article) 


Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula

Lu Han, Caizhen Liang, Xican Li.

Cited by (1)

Abstract
As a tea or functional vegetable in southeast China, Begonia fimbristipula Hance possesses various pharmacological effects including antioxidant ability. However, its antioxidant effect has not been reported yet. The objective of this work was to investigate its antioxidant ability, then further discuss the antioxidant mechanism. B.fimbristipula Hance was extracted by methanol to obtain methanol extract of B.fimbristipula Hance (EBFH). EBFH was then measured by various antioxidant methods, including DNA damage assay, 2,2-Diphenyl-1-picrylhydrazyl radical (DPPH•) assay, superoxide anion radical (O2• ) scavenging assay, 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate radical (ABTS•+) assay, Fe3+-reducing assay and Cu2+-reducing assay. The total phenolic content of EBFH was estimated using Folin-Ciocalteu method as well. Our results revealed that EBFH could effectively protect against hydroxyl-induced DNA damage. In addition, it could also scavenge O2• , DPPH• and ABTS•+ radicals, and reduce Fe3+ and Cu2+. The total phenols content of EBFH was 133.25 2.88 pyrogallol/g. In conclusion, B.fimbristipula Hance can effectively protect against hydroxyl-induced DNA damage. One mechanism of this protective effect may be radical scavenging via donating hydrogen atom (H•), donating electron (e). Its antioxidant ability may be mainly attributed to the total phenols, especially cyanidin-3-O-glucoside.

Key words: Begonia fimbristipula; DNA damage; Hydroxyl radical


 
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This Article Cited By the following articles

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Applications in Plant Sciences 2016; 4(7): 1600004.

1
 

How to Cite this Article
Pubmed Style

Han L, Liang C, Li X. Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxid Antioxid Med Sci. 2013; 2(3): 195-200. doi:10.5455/oams.210713.or.049


Web Style

Han L, Liang C, Li X. Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. http://www.ejmoams.com/?mno=38194 [Access: August 20, 2018]. doi:10.5455/oams.210713.or.049


AMA (American Medical Association) Style

Han L, Liang C, Li X. Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxid Antioxid Med Sci. 2013; 2(3): 195-200. doi:10.5455/oams.210713.or.049



Vancouver/ICMJE Style

Han L, Liang C, Li X. Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxid Antioxid Med Sci. (2013), [cited August 20, 2018]; 2(3): 195-200. doi:10.5455/oams.210713.or.049



Harvard Style

Han, L., Liang, C. & Li, X. (2013) Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxid Antioxid Med Sci, 2 (3), 195-200. doi:10.5455/oams.210713.or.049



Turabian Style

Han, Lu, Caizhen Liang, and Xican Li. 2013. Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxidants and Antioxidants in Medical Science, 2 (3), 195-200. doi:10.5455/oams.210713.or.049



Chicago Style

Han, Lu, Caizhen Liang, and Xican Li. "Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula." Oxidants and Antioxidants in Medical Science 2 (2013), 195-200. doi:10.5455/oams.210713.or.049



MLA (The Modern Language Association) Style

Han, Lu, Caizhen Liang, and Xican Li. "Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula." Oxidants and Antioxidants in Medical Science 2.3 (2013), 195-200. Print. doi:10.5455/oams.210713.or.049



APA (American Psychological Association) Style

Han, L., Liang, C. & Li, X. (2013) Protective affect against hydroxyl radical induced DNA damage and antioxidant mechanism of Begonia fimbristipula. Oxidants and Antioxidants in Medical Science, 2 (3), 195-200. doi:10.5455/oams.210713.or.049





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