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Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice

Ehsanollah Sakhaee, Jalil Abshenas, Reza Kheirandish, Omid Azari, Arefeh Karamshahi.

Cited by (3)

Abstract
This study was set to investigate whether the adverse effects of long-term copper (Cu) consumption on testicular tissue could be prevented by vitamin C administration. Thirty-six sexually mature male NMRI mice were randomly divided into three groups of 12 animals. The first group (group Cu) which is treated by gavage with copper sulfate at a dose of 200 mg/kg/day (0.2 ml) and 0.1 ml of distilled water via intraperitoneal (i.p.) injection daily for 6 weeks, the treatment group (group Cu + Vit.C) which is treated by gavage with copper sulfate at a dose of 200 mg/kg/day (0.2 ml) and received 10 mg/kg/day (0.1 ml) vitamin C by i.p. injection, and the control group which received the same volume of distilled water by gavage and i.p. injection during the experimental period. Shrinkage and collapse of tubules, decreased number of germinal cells and decrease in epithelial height were histopathologial lesions of testes of Cu group, while, treatment group showed normal morphology with presence of spermatozoa in their lumen. In conclusion, vitamin C can be one of the suitable choices for preventive therapeutic effects and/or improvement in testicular tissue after Cu toxicosis.

Key words: Copper poisoning; Histopathology; Mice; Testes; Vitamin C


 
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This Article Cited By the following articles

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Asian Pacific Journal of Reproduction 2016; 5(1): 46.

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How to Cite this Article
Pubmed Style

Sakhaee E, Abshenas J, Kheirandish R, Azari O, Karamshahi A. Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxid Antioxid Med Sci. 2013; 2(2): 125-129. doi:10.5455/oams.030413.or.035


Web Style

Sakhaee E, Abshenas J, Kheirandish R, Azari O, Karamshahi A. Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. http://www.ejmoams.com/?mno=33290 [Access: February 20, 2018]. doi:10.5455/oams.030413.or.035


AMA (American Medical Association) Style

Sakhaee E, Abshenas J, Kheirandish R, Azari O, Karamshahi A. Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxid Antioxid Med Sci. 2013; 2(2): 125-129. doi:10.5455/oams.030413.or.035



Vancouver/ICMJE Style

Sakhaee E, Abshenas J, Kheirandish R, Azari O, Karamshahi A. Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxid Antioxid Med Sci. (2013), [cited February 20, 2018]; 2(2): 125-129. doi:10.5455/oams.030413.or.035



Harvard Style

Sakhaee, E., Abshenas, J., Kheirandish, R., Azari, O. & Karamshahi, A. (2013) Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxid Antioxid Med Sci, 2 (2), 125-129. doi:10.5455/oams.030413.or.035



Turabian Style

Sakhaee, Ehsanollah, Jalil Abshenas, Reza Kheirandish, Omid Azari, and Arefeh Karamshahi. 2013. Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxidants and Antioxidants in Medical Science, 2 (2), 125-129. doi:10.5455/oams.030413.or.035



Chicago Style

Sakhaee, Ehsanollah, Jalil Abshenas, Reza Kheirandish, Omid Azari, and Arefeh Karamshahi. "Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice." Oxidants and Antioxidants in Medical Science 2 (2013), 125-129. doi:10.5455/oams.030413.or.035



MLA (The Modern Language Association) Style

Sakhaee, Ehsanollah, Jalil Abshenas, Reza Kheirandish, Omid Azari, and Arefeh Karamshahi. "Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice." Oxidants and Antioxidants in Medical Science 2.2 (2013), 125-129. Print. doi:10.5455/oams.030413.or.035



APA (American Psychological Association) Style

Sakhaee, E., Abshenas, J., Kheirandish, R., Azari, O. & Karamshahi, A. (2013) Histopathological evaluation of supportive effect of vitamin C on testes following long-term administration of copper sulfate in mice. Oxidants and Antioxidants in Medical Science, 2 (2), 125-129. doi:10.5455/oams.030413.or.035





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