ISSN 2146-8389
 

Original Research (Original Article) 


Evaluation of analgesic and antioxidant potential of ethanolic extract of <i>Nymphaea alba</i> rhizome

Anindya Bose, Sarbani Dey Ray, Moumita Sahoo.

Cited by (4)

Abstract
Nymphaea alba is rich in phytoconstituents that posses pharmacological actions. But any scientific literature about its antioxidant and analgesic actions using its rhizome part is not documented. So the objective of the present research is focused to evaluate the analgesic activity and antioxidant potential by different models of scavenging properties of N.alba extract. Nymphaea alba rhizome ethanol extract was investigated for their analgesic activity by acetic acid- and formalin-induced analgesia and antioxidant activities employing 3 in vitro assay systems, i.e. 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO) and superoxide anion radicals scavenging. The reducing properties and total phenolic content was also observed. The extract at a dose of 600 mg/kg was found more potent in acetic acid induced pain and showed more licking activity in both the phases of formalin acid induced pain than the standard drug. IC50 values for DPPH, NO and superoxide radical-scavenging activity were 63.9, 49.21 and 79.56 μg/ml, respectively. The IC50 of extract is more comparable to the standard drug for scavenging superoxide anion than the other methods. The antioxidant activities of N.alba ethanolic extract was moderate in comparison to the standard antioxidant ascorbic acid, achieved by the scavenging ability observed against DPPH, NO radicals and superoxide anions. The antioxidant effects observed in this study may be due to the presence of tannins and phenolic compounds. However, unless a thorough compositional analysis of the extract along with activity correlation is not carried out there can be no statements with high degree of certainty.

Key words: Analgesic; Antioxidant; Phenolics; Tannins; Radicals


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Anindya Bose
Articles by Sarbani Dey Ray
Articles by Moumita Sahoo
on Google
on Google Scholar
Article Statistics
 Viewed: 3983
Downloaded: 1044
Cited: 4

REFERENCES
1.Valko M, Leibfritz D, Moncol J, Cronin M, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39:44-84.

http://dx.doi.org/10.1016/j.biocel.2006.07.001

[Pubmed]   


2.Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol 1997; 82:291-5.

[
Pubmed]   


3.Jayaprakash GK, Rao LJ. Phenolic constituents from lichen Parmontrema stuppeum (Nyl.) Hale and their antioxidant activity. Z Naturforsch 2000; 55:1018-22.


4.Corrado B, Marco T, Colucci R, Fornai M, Antonioli L, Ghisu N, Tacca MD. Role of coxibs in the strategies for gastrointestinal protection in patients requiring chronic non-steroidal anti inflammatory therapy. Pharm Res 2009; 59:90-100.

http://dx.doi.org/10.1016/j.phrs.2008.11.004

[
Pubmed]   


5.Eliana R, Ricardo T, Jose C, Galduroz F, Giuseppina N. Plants with possible anxiolytic and/or hypnotic effects indicated by three brazilian cultures - indians, afrobrazilians, and river-dwellers. Studies in Natural Products Chemistry 2008; 35:549-95.

http://dx.doi.org/10.1016/S1572-5995(08)80014-2


6.Adnaik RS, Pai PT, Sapakal VD, Naikwade NS, Magdum CS. Anxiolytic activity of Vitex negundo Linn in experimental models of anxiety in mice. Int J Green Pharm 2009; 3:243-47.

http://dx.doi.org/10.4103/0973-8258.56284


7.Robin D. Nymphaea odorata: White pond lily. Medical Herbalism 2001; 11:6-7.


8.Vergeera LH, Vander VG. Phenolic content of daylight-exposed and shaded floating leaves of water lilies (Nymphaeaceae) in relation to infection by fungi. Oecologia 1997; 112:481-4.

http://dx.doi.org/10.1007/s004420050335


9.Duke JA. Duke's Handbook of Medicinal Plants of the Bible. CRC Press, Boca Raton, FL, USA, pp 302-305, 2008.

http://dx.doi.org/10.1201/9781420043174


10.Khan N, Sultana S. Anticarcinogenic effect of Nymphaea alba against oxidative damage and hyperproliferative response and renal carcinogenis in Wistar rats. Mol Cell Biochem 2005; 271:1-11.

http://dx.doi.org/10.1007/s11010-005-2258-2

[
Pubmed]   


11.Naghma K, Sarwat S. Inhibition of potassium bromate-induced renal oxidative stress and hyperproliferative response by Nymphaea alba in Wistar rats. J Enzyme Inhib Med Chem 2005; 20:275-83.

http://dx.doi.org/10.1080/14756360400028119

[
Pubmed]   


12.Bagul M, Srinivasa H, Padh H, Rajani M. A rapid densitometric method for simultaneous quantification of gallic acid and ellagic acid in herbal raw materials using HPTLC. J Sep Sci 2005; 28:581-4.

http://dx.doi.org/10.1002/jssc.200301695

[
Pubmed]   


13.Madhusudhan N, Lakshmi T, Gowtham KS, Ramakrishanan, Konda VGR, Roy A, Geetha RV. Invitro antioxidant and free radical scavenging activity of aqueous and ethanolic flower extract of Nymphaea alba. Int J Drug Dev Res 2011; 3:23-6.


14.Evans WC. Trease and Evans Pharmacognosy. 14th edition, WB Sauders, London, UK, pp 56-58, 1996.


15.Sawadogo WR, Boly R, Lompo M, Some N, Lamien CE, Guissou IP, Nacoulma OG. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol 2006; 2:435-8.

http://dx.doi.org/10.3923/ijp.2006.435.438


16.Wibool R, Sae WC, Reanmongkol W, Wongnawaa M. Antinociceptive activity of the methanolic extract of Kaempferia galangalLinn. in experimental animals. J Ethnopharmacol 2008; 118: 225-30.

http://dx.doi.org/10.1016/j.jep.2008.04.002

[
Pubmed]   


17.Koduru S, Jimoh FO, Grierson DS, Afolayan AJ. Antioxidant activity of twosteroid alkaloids extracted from Solanum aculeastrum. J Pharmacol Toxicol 2007; 2:160-7.

http://dx.doi.org/10.3923/jpt.2007.160.167


18.Singelton VR, Orthifer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999; 299:152-78.

http://dx.doi.org/10.1016/S0076-6879(99)99017-1


19.Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001; 64:892-5.

http://dx.doi.org/10.1021/np0100845

[
Pubmed]   


20.Govindarajan R, Rastogi S, Vijayakumar M, Shirwaikar A, Rawat AK, Mehrotra S, Pushpangadan P. Studies on antioxidant activities of Desmodium gangeticum. Biol Pharm Bull 2003; 26:1424-425.

http://dx.doi.org/10.1248/bpb.26.1424

[
Pubmed]   


21.Nishikimi M, Appaji N, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazinemethosulphate and molecular oxygen. Biochem Biophys Res Commun 1972; 46:849-54.

http://dx.doi.org/10.1016/S0006-291X(72)80218-3

[
Pubmed]   


22.Svilaas A, Sakhi AK, Andersen LF, Svilaas T, Strom EC, Jacobs DR, Ose L, Blomhoff R. Intakes of antioxidants in coffee, wine and vegetables are correlated with plasma carotenoids in humans. J Nutr 2004; 134:562-7.

[
Pubmed]   


23.Parke DV, Sapota A. Chemical toxicity and reactive species. Int J Occup Med Environ Health 1996; 9:119-23.


24.Rahman MAA, Moon SS. Antioxidant polyphenol glycosides from the Plant Draba nemorosa. Bull Korean Chem Soc 2007; 28:827-31.

http://dx.doi.org/10.5012/bkcs.2007.28.5.827


25.Kok CJ, Hof CHJ, Lenssen JPM, van der Velde G.The influence of pH on concentrations of protein and phenolics and resource quality of decomposing floating leaf material of Nymphaea alba L. (Nymphaeaceae) for the detritivore Asellus aquaticus (L.). Oecologia 1992; 91:229-34.

http://dx.doi.org/10.1007/BF00317788


26.Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, Riechel TL. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agric Food Chem 1998; 46:1887-92.

http://dx.doi.org/10.1021/jf970975b


27.Eldahshan OA, Ayoub NA, Singab ANB, Al-Azizi MM. Potential superoxide anion radical scavenging activity of Doum Palm (Hyphaene thebaica L.) leaves extract. Rec Nat Prod 2008; 2:83-93.


28.Kaur P, Arora S. Superoxide anion radical scavenging activity of Cassia siamea and Cassia javanica. Med Chem Res 2011; 20: 9-15.

http://dx.doi.org/10.1007/s00044-009-9274-9


This Article Cited By the following articles

Characterization of the bioactive constituents of Nymphaea alba rhizomes and evaluation of anti-biofilm as well as antioxidant and cytotoxic properties
J. Med. Plants Res. 2016; 10(26): 390.

1
 
Scientific evaluation of medicinal plants used for the treatment of abnormal uterine bleeding by Avicenna
Archives of Gynecology and Obstetrics 2015; 292(1): 21-35.

2
 
Anti-inflammatory and anti-oxidative effects of herbal preparation EM 1201 in adjuvant arthritic rats
Medicina 2015; 51(6): 368-377.

3
 
An Evidence-based Review on Medicinal Plants used for the Treatment of Peptic Ulcer in Traditional Iranian Medicine
International Journal of Pharmacology 2013; 9(2): 108-124.

4
 

How to Cite this Article
Pubmed Style

Bose A, Ray SD, Sahoo M. Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxid Antioxid Med Sci. 2012; 1(3): 217-223. doi:10.5455/oams.140912.or.017


Web Style

Bose A, Ray SD, Sahoo M. Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. http://www.ejmoams.com/?mno=23508 [Access: August 20, 2018]. doi:10.5455/oams.140912.or.017


AMA (American Medical Association) Style

Bose A, Ray SD, Sahoo M. Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxid Antioxid Med Sci. 2012; 1(3): 217-223. doi:10.5455/oams.140912.or.017



Vancouver/ICMJE Style

Bose A, Ray SD, Sahoo M. Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxid Antioxid Med Sci. (2012), [cited August 20, 2018]; 1(3): 217-223. doi:10.5455/oams.140912.or.017



Harvard Style

Bose, A., Ray, S. D. & Sahoo, M. (2012) Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxid Antioxid Med Sci, 1 (3), 217-223. doi:10.5455/oams.140912.or.017



Turabian Style

Bose, Anindya, Sarbani Dey Ray, and Moumita Sahoo. 2012. Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxidants and Antioxidants in Medical Science, 1 (3), 217-223. doi:10.5455/oams.140912.or.017



Chicago Style

Bose, Anindya, Sarbani Dey Ray, and Moumita Sahoo. "Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome." Oxidants and Antioxidants in Medical Science 1 (2012), 217-223. doi:10.5455/oams.140912.or.017



MLA (The Modern Language Association) Style

Bose, Anindya, Sarbani Dey Ray, and Moumita Sahoo. "Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome." Oxidants and Antioxidants in Medical Science 1.3 (2012), 217-223. Print. doi:10.5455/oams.140912.or.017



APA (American Psychological Association) Style

Bose, A., Ray, S. D. & Sahoo, M. (2012) Evaluation of analgesic and antioxidant potential of ethanolic extract of Nymphaea alba rhizome. Oxidants and Antioxidants in Medical Science, 1 (3), 217-223. doi:10.5455/oams.140912.or.017





Most Viewed Articles
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Effects of silymarin, N-acetylcysteine and selenium in the treatment of papulopustular acne
    Haidar Hamid Al-Anbari, Ahmed Salih Sahib, Ahmed R. Abu Raghif
    Oxid Antioxid Med Sci. 2012; 1(3): 201-207
    » Abstract & References » doi: 10.5455/oams.290912.or.019

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043

  • Most Downloaded
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica
    Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano
    Oxid Antioxid Med Sci. 2015; 4(1): 49-55
    » Abstract & References » doi: 10.5455/oams.180415.or.084

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Protective properties of complex of quercetin, selenium, catechins and curcumin against DNA damage
    Jana Kadrabova, Marica Krajcovicova-Kudlackova, Alexander Madaric, Martina Valachovicova, Csilla Mislanova, Maria Korenovska
    Oxid Antioxid Med Sci. 2012; 1(3): 179-184
    » Abstract & References » doi: 10.5455/oams.180912.or.018

  • Airway antioxidant capacity and pH in chronic obstructive pulmonary disease
    Wei Lee, Hsien Loo, Paul S. Thomas
    Oxid Antioxid Med Sci. 2012; 1(3): 153-160
    » Abstract & References » doi: 10.5455/oams.300812.or.016

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001

  • Most Cited Articles
  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009
    Cited : 13 times [Click to see citing articles]

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038
    Cited : 11 times [Click to see citing articles]

  • Role of free radicals and antioxidants in gynecological cancers: current status and future prospects
    Lokanatha Valluru, Subramanyam Dasari, Rajendra Wudayagiri
    Oxid Antioxid Med Sci. 2014; 3(1): 15-26
    » Abstract & References » doi: 10.5455/oams.201113.rv.011
    Cited : 10 times [Click to see citing articles]

  • Silymarin and naringenin protects nicotine induced oxidative stress in young rats
    Anshu Jain, Nidhi Dwivedi, Rakesh Bhargava, Swaran J.S. Flora
    Oxid Antioxid Med Sci. 2012; 1(1): 41-49
    » Abstract & References » doi: 10.5455/oams.130412.or.004
    Cited : 9 times [Click to see citing articles]

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043
    Cited : 9 times [Click to see citing articles]

  • Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats
    Fatmah Ali Matough, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, Jamaludin Mohamed
    Oxid Antioxid Med Sci. 2012; 1(1): 59-68
    » Abstract & References » doi: 10.5455/oams.300412.or.006
    Cited : 8 times [Click to see citing articles]

  • Peroxidative index as novel marker of hydrogen peroxide involvement in lipid peroxidation from coal dust exposure
    Nia Kania, Bambang Setiawan, Edi Widjajanto, Nurdiana Nurdiana, M. Aris Widodo, H.M.S. Chandra Kusuma
    Oxid Antioxid Med Sci. 2012; 1(3): 209-215
    » Abstract & References » doi: 10.5455/oams.031012.or.020
    Cited : 8 times [Click to see citing articles]

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051
    Cited : 7 times [Click to see citing articles]

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001
    Cited : 6 times [Click to see citing articles]

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005
    Cited : 6 times [Click to see citing articles]