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Antioxidant and antimicrobial profile of a secondary metabolite of <i>Pseudomonas aeruginosa</i>: ESR and spectrophotometric method

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam.

Abstract
Marine <i>Pseudomonas</i> is a potential provider of novel bioactive metabolites and currently emerged as an important source for natural products with unique chemical diversity. The aim of the present study was to evaluate antimicrobial and antioxidant potential of partially purified fraction III extracted as a secondary metabolite from <i>Pseudomonas aeruginosa</i> MTCC 5210 isolated from marine sediments of coastal Tamil Nadu, India. In the present study, the antioxidant property of fraction III was evaluated using spectrophotometric and alectron spin resonance (ESR) techniques. The results of these studies revealed, fraction III quenches 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH●), nitric oxide radical and superoxide radical at appreciable levels. The ESR measurements show the reduction in peak intensities of DPPH● and nitric oxide radical after the addition of fraction III. It was also observed that quenching and reduction in peak intensities of standard free radicals studied are in proportion to the concentrations of fraction III studied. The antimicrobial activity of fraction III was evaluated using antibiotic resistant microbes such as methicillin resistant <i>Staphylococcus aureus</i> (MRSA). Since there is a constant demand for the natural antioxidants, the results of our present study suggest, that fraction III may act as a potential antioxidant and antibacterial compound.

Key words: Antibacterial activity; DPPH●; ESR; Nitric oxide; <i>Pseudomonas aeruginosa</i>; Superoxide


 
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How to Cite this Article
Pubmed Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. 2012; 1(2): 119-126. doi:10.5455/oams.200812.or.013

Web Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. http://www.ejmoams.com/?mno=22582 [Access: November 16, 2018]. doi:10.5455/oams.200812.or.013

AMA (American Medical Association) Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. 2012; 1(2): 119-126. doi:10.5455/oams.200812.or.013


Vancouver/ICMJE Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. (2012), [cited November 16, 2018]; 1(2): 119-126. doi:10.5455/oams.200812.or.013


Harvard Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam (2012) Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci, 1 (2), 119-126. doi:10.5455/oams.200812.or.013


Turabian Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. 2012. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxidants and Antioxidants in Medical Science, 1 (2), 119-126. doi:10.5455/oams.200812.or.013


Chicago Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. "Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method." Oxidants and Antioxidants in Medical Science 1 (2012), 119-126. doi:10.5455/oams.200812.or.013


MLA (The Modern Language Association) Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam. "Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method." Oxidants and Antioxidants in Medical Science 1.2 (2012), 119-126. Print. doi:10.5455/oams.200812.or.013


APA (American Psychological Association) Style

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam (2012) Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxidants and Antioxidants in Medical Science, 1 (2), 119-126. doi:10.5455/oams.200812.or.013




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