ISSN 2146-8389
 

Original Research (Original Article) 


Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition

Marica Krajcovicova-Kudlackova, Martina Valachovicova, Csilla Mislanova, Jana Pribojova.

Cited by (1)

Abstract
Nutrition is a key environmental factor influencing the incidence of chronic age related free radical induced diseases. The better antioxidant status by a sufficient consumption of food commodities with antioxidant compounds helps to minimize oxidative damage, and thus can delay or prevent pathological changes. In three groups of adult apparently healthy non-smoking population of different nutritional habits (93 persons of general population, non-vegetarians, NV; 77 semi-vegetarians, V-S; 64 lacto-ovo-vegetarians, V-LO) plasma concentrations of vitamins B9, C, E and β-carotene, potential prooxidant homocysteine and products of oxidative damage of lipids (total peroxides, malondialdehyde, oxidized LDL) as well as and oxidative DNA damage in lymphocytes (strand breaks, oxidized purines, oxidized pyrimidines) were measured. Vegetarian (V-S, V-LO) vs NV plasma concentrations of vitamin C, vitamin E, lipid standardized vitamin E, β-carotene and vitamin B9 were significantly higher. Plasma total homocysteine values are significantly higher in both vegetarian groups as a consequence of deficient vitamin B12 concentrations. Hyperhomocysteinemia, a marker of systemic or endothelial oxidative stress may promote the production of hydroxyl radicals which are the initiators of lipid oxidation. In spite of 28.5% and 40.6% incidence of hyperhomocysteinemia in V-S and V-LO, the concentrations of total peroxides, malondialdehyde as well as values of oxidized LDL were significantly reduced as a consequence of a better antioxidant status. Parameters of DNA damage as biomarkers of cancer risk in epidemiological studies are significantly reduced in two groups of vegetarians. The findings suggest that dominant consumption of plant food in vegetarians, higher intake of dietary antioxidants and their higher plasma concentrations in comparison to general population may have protective effects against oxidative damage of molecules and a preventive effect on incidence of chronic age related diseases.

Key words: Antioxidant vitamins; DNA oxidation; Lipid peroxidation; Oxidative damage; General population; Vegetarians


 
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REFERENCES
1.Bonnefoy M, Drai J, Kostka T. Antioxidants to slow aging, facts and perspectives. Presse Med 2002; 31:1174-84.

[Pubmed]   

2.Gutteridge JM, Halliwell B. Antioxidants: Molecules, medicines, and myths. Biochem Biophys Res Comm 2010; 393:561-4.

http://dx.doi.org/10.1016/j.bbrc.2010.02.071

[
Pubmed]   

3.Gey KF. Cardiovascular disease and vitamins. Concurrent correction of ‘suboptimal’ plasma antioxidant levels may, as important part of ‘optimal’ nutrition, help to prevent early stages of cardiovascular disease and cancer, respectively. Bibl Nutr Dieta 1995; 52:75-91.

[
Pubmed]   

4.Krajcovicova-Kudlackova M, Spustova V, Paukova V. Lipid peroxidation and nutrition. Physiol Res 2004; 53:219-24.

[
Pubmed]   

5.Kapiszewska M. A vegetable to meat consumption ratio as a relevant factor determining cancer preventive diet. The Mediterranean versus other European countries. Forum Nutr 2006; 59:130-53.

http://dx.doi.org/10.1159/000095211

[
Pubmed]   

6.Key TJ, Appleby PN, Rosell MS. Health effects of vegetarian and vegan diets. Proc Nutr Soc 2006; 65:35-41.

http://dx.doi.org/10.1079/PNS2005481

[
Pubmed]   

7.Krajcovicova-Kudlackova M, Paukova V, Bacekova M, Dusinska M. Lipid Peroxidation in relation to vitamin C and vitamin E levels. Cent Eur J Publ Health 2004; 12:46-8.

[
Pubmed]   

8.Krajcovicova-Kudlackova M, Dusinska M, Valachovicova M, Blazicek P, Paukova V. Products of DNA, protein and lipid oxidative damage in relation to vitamin C plasma concentration. Physiol Res 2006; 55:227-31.

[
Pubmed]   

9.Cerhata D, Bauerova A, Ginter E. Determination of ascorbic acid in blood serum using high-performance liquid chromatography and its correlation with spectrophotometric (colorimetric) detection. Ces Slov Farm 1994; 43:166-8.

[
Pubmed]   

10.Lee BL, Chua SC, Ong HY, Ong CN. High-performance liquid chromatographic method for routine determination of vitamins A and E, and beta-carotene in plasma. J Chromatogr 1992; 581:41-7.

[
Pubmed]   

11.Houze P, Gamra S, Madelaine I, Bousquet B, Gourmel B. Simultaneous determination of total plasma glutathione, homocysteine, cysteinylglycine, and methionine by high performance liquid chromatography with electrochemical detection. J Clin Lab Anal 2001; 15:144-53.

http://dx.doi.org/10.1002/jcla.1018

[
Pubmed]   

12.Wong SHY, Knight JA, Hopfer SM, Zaharia O, Leach CHN, Sunderman FW. Lipoperoxidates in plasma as measured by liquid chromatographic separation of malondialdehyde-thiobarbituric acid adduct. Clin Chem 1987; 33:214-20.

[
Pubmed]   

13.Collins AR, Dusinska M, Gedik CM, Stetina R. Oxidative damage to DNA, do we have a reliable biomarker? Environ Health Perspect 1996; 104:465-9.

[
Pubmed]    [PMC Free Fulltext]   

14.Dusinska M, Collins A. Detection of oxidized purines and UV-induced photoproducts in DNA of single cells by inclusion of lesion-specific enzymes in the comet assay. Altern Lab Anim 1996; 24:405-11.


15.Waldmann A, Koschitzke JW, Leitzmann C, Hahn A. Dietary intakes and blood concentrations of antioxidant vitamins in German vegans. Int J Vitam Nutr Res 2005; 75:28-36.

http://dx.doi.org/10.1024/0300-9831.75.1.28

[
Pubmed]   

16.Stanger O, Wonisch W. Enzymatic and non-enzymatic antioxidative effects of folic acid and its reduced derivates. Subcell Biochem 2012; 56:131-51.

http://dx.doi.org/10.1007/978-94-007-2199-9_8

[
Pubmed]   

17.Krajcovicova-Kudlackova M, Blazicek P, Kopcova J, Bederova A, Babinska K. Homocysteine levels in vegetarians versus omnivores. Ann Nutr Metab 2000; 44:135-8.

http://dx.doi.org/10.1159/000012827

[
Pubmed]   

18.Cacciapuoti F. Hyper-homocysteinemia: a novel risk factor or a powerful marker for cardiovascular disease? Pathogenetic and therapeutical uncertainties. J Thromb Thrombol 2011; 32:82-8.

http://dx.doi.org/10.1007/s11239-011-0550-4

[
Pubmed]   

19.Hoffman M. Hypothesis: hyperhomocysteinemia is an indicator of oxidant stress. Med Hypoptheses 2011; 77:1088-93.

http://dx.doi.org/10.1016/j.mehy.2011.09.009

[
Pubmed]   

20.Caraffa A, Anogeianaki A, Angelucci D, Cianchetti E, D’Alessandro M, Maccauro G, Salini V, Caraffa A, Tete S, Conti F, Tripodi D, Fulcheri M, Frydas S, Shaik-Dasthagirisaheb YB. Cholesterol and vitamins, revisited study. J Biol Regul Homeost Agents 2011; 25:505-15.

[
Pubmed]   

21.Bjelakovic G, Nikolova D, Simonetti RG, Gluud C. Systematic review: primary and secondary prevention of gastrointestinal cancers with antioxidant supplements. Aliment Pharmacol Ther 2008; 28:689-703.

http://dx.doi.org/10.1111/j.1365-2036.2008.03785.x

[
Pubmed]   

This Article Cited By the following articles

Values of lipid and DNA damage in dependence to smoking and plasma antioxidant concentrations
Oxidants and Antioxidants in Medical Science 2013; (): 1.

1
 

How to Cite this Article
Pubmed Style

Krajcovicova-Kudlackova M, Valachovicova M, Mislanova C, Pribojova J. Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxid Antioxid Med Sci. 2012; 1(2): 147-151. doi:10.5455/oams.130812.or.011


Web Style

Krajcovicova-Kudlackova M, Valachovicova M, Mislanova C, Pribojova J. Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. http://www.ejmoams.com/?mno=21537 [Access: August 20, 2018]. doi:10.5455/oams.130812.or.011


AMA (American Medical Association) Style

Krajcovicova-Kudlackova M, Valachovicova M, Mislanova C, Pribojova J. Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxid Antioxid Med Sci. 2012; 1(2): 147-151. doi:10.5455/oams.130812.or.011



Vancouver/ICMJE Style

Krajcovicova-Kudlackova M, Valachovicova M, Mislanova C, Pribojova J. Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxid Antioxid Med Sci. (2012), [cited August 20, 2018]; 1(2): 147-151. doi:10.5455/oams.130812.or.011



Harvard Style

Krajcovicova-Kudlackova, M., Valachovicova, M., Mislanova, C. & Pribojova, J. (2012) Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxid Antioxid Med Sci, 1 (2), 147-151. doi:10.5455/oams.130812.or.011



Turabian Style

Krajcovicova-Kudlackova, Marica, Martina Valachovicova, Csilla Mislanova, and Jana Pribojova. 2012. Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxidants and Antioxidants in Medical Science, 1 (2), 147-151. doi:10.5455/oams.130812.or.011



Chicago Style

Krajcovicova-Kudlackova, Marica, Martina Valachovicova, Csilla Mislanova, and Jana Pribojova. "Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition." Oxidants and Antioxidants in Medical Science 1 (2012), 147-151. doi:10.5455/oams.130812.or.011



MLA (The Modern Language Association) Style

Krajcovicova-Kudlackova, Marica, Martina Valachovicova, Csilla Mislanova, and Jana Pribojova. "Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition." Oxidants and Antioxidants in Medical Science 1.2 (2012), 147-151. Print. doi:10.5455/oams.130812.or.011



APA (American Psychological Association) Style

Krajcovicova-Kudlackova, M., Valachovicova, M., Mislanova, C. & Pribojova, J. (2012) Antioxidative vitamins and oxidative lipid and DNA damage in relation to nutrition. Oxidants and Antioxidants in Medical Science, 1 (2), 147-151. doi:10.5455/oams.130812.or.011





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