ISSN 2146-8389
 

Original Research (Original Article) 


Antioxidant role of plumbagin in modification of radiation-induced oxidative damage

Ramachandran Gangabhagirathi, Ravi Joshi.

Abstract
Objective: Plumbagin is a well-known ingredient of Plumbago zeylanica, which is used in Ayurveda for its multiple therapeutic actions. We have attempted to understand protective effects of plumbagin against gamma radiation-induced damage to lipids, proteins, and enzymes of rat liver mitochondria in vitro.
Methods: Rat liver mitochondrial preparation of female Wistar rats has been exposed to gamma radiation (450 Gy) to induce oxidative damage of its components. The protection offered by increasing concentration of plumbagin during irradiation for lipids, proteins and enzymes of rat liver mitochondrial preparation has been studied. The protection exerted by plumbagin (50-150 M) has been studied using different standard assays for lipids (thiobarbituric acid reactive substance and ferrous oxidation in xylenol orange), proteins (dinitrophenylhydrazine and dithiobisnitrobenzoic acid), glutathione (fluorescence), superoxide dismutase (epinephrine oxidation) and succinate dehydrogenase (dichlorophenolindophenol reduction).
Results: Plumbagin has been found to inhibit lipid peroxidation and protein carbonyl formation; protect thiols (glutathione and protein) and enzyme activity (superoxide dismutase and succinate dehydrogenase) in a concentration dependent manner.
Conclusion: The protection provided by plumbagin to lipids, proteins and enzymes of rat liver mitochondrial preparation against gamma radiation-induced damage suggest that it can provide radioprotection in the living systems.

Key words: Gamma radiation, lipid peroxidation, plumbagin, protein damage


 
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How to Cite this Article
Pubmed Style

Ramachandran Gangabhagirathi, Ravi Joshi. Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxid Antioxid Med Sci. 2015; 4(2): 85-90. doi:10.5455/oams.260415.or.085


Web Style

Ramachandran Gangabhagirathi, Ravi Joshi. Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. http://www.ejmoams.com/?mno=177380 [Access: November 16, 2018]. doi:10.5455/oams.260415.or.085


AMA (American Medical Association) Style

Ramachandran Gangabhagirathi, Ravi Joshi. Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxid Antioxid Med Sci. 2015; 4(2): 85-90. doi:10.5455/oams.260415.or.085



Vancouver/ICMJE Style

Ramachandran Gangabhagirathi, Ravi Joshi. Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxid Antioxid Med Sci. (2015), [cited November 16, 2018]; 4(2): 85-90. doi:10.5455/oams.260415.or.085



Harvard Style

Ramachandran Gangabhagirathi, Ravi Joshi (2015) Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxid Antioxid Med Sci, 4 (2), 85-90. doi:10.5455/oams.260415.or.085



Turabian Style

Ramachandran Gangabhagirathi, Ravi Joshi. 2015. Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxidants and Antioxidants in Medical Science, 4 (2), 85-90. doi:10.5455/oams.260415.or.085



Chicago Style

Ramachandran Gangabhagirathi, Ravi Joshi. "Antioxidant role of plumbagin in modification of radiation-induced oxidative damage." Oxidants and Antioxidants in Medical Science 4 (2015), 85-90. doi:10.5455/oams.260415.or.085



MLA (The Modern Language Association) Style

Ramachandran Gangabhagirathi, Ravi Joshi. "Antioxidant role of plumbagin in modification of radiation-induced oxidative damage." Oxidants and Antioxidants in Medical Science 4.2 (2015), 85-90. Print. doi:10.5455/oams.260415.or.085



APA (American Psychological Association) Style

Ramachandran Gangabhagirathi, Ravi Joshi (2015) Antioxidant role of plumbagin in modification of radiation-induced oxidative damage. Oxidants and Antioxidants in Medical Science, 4 (2), 85-90. doi:10.5455/oams.260415.or.085





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