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In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain

Olusola B. Adewale, Amos Onasanya, Adewale O. Fadaka, Hilda Iwere, Scholastica O. Anadozie, Olukemi A. Osukoya, Israel I. Olayide.

Abstract
Objective: Antioxidants are known to offer resistance against oxidative stress by scavenging free radicals, inhibiting lipid peroxidation and by other mechanisms. They therefore help in preventing the free radicals induced diseases. Solanum macrocarpon is an important medicinal plant with rich source of nutritional and medicinal constituents. The objective of the present study was to investigate the ability of S.macrocarpon leaves to protect tissues against iron (Fe2+)-induced lipid peroxidation in rat’s liver and brain.
Methods: The total phenolic and total flavonoid contents of the aqueous extract of S.macrocarpon leaves, as well as its antioxidant potential, free radical scavenging activity, reducing power and protective action on tissues against lipid peroxidation were carried out.
Results: S.macrocarpon leaves revealed high phenolic and flavonoid content. Incubating the liver and brain of rats with S.macrocarpon leave extract exhibited high percentage inhibition of lipid peroxidation induced by iron(II) sulphate. Moreover, DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity and reducing power of the extract increased as the concentration increases.
Conclusion: The aqueous extract of Solanum macrocarpon leaves possess a powerful antioxidant activity and can offer good protection against oxidative damage to body cells, especially liver and brain.

Key words: Lipid peroxidation; Iron(II) sulphate; Reducing power; Solanum macrocarpon


 
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How to Cite this Article
Pubmed Style

Adewale OB, Onasanya A, Fadaka AO, Iwere H, Anadozie SO, Osukoya OA, Olayide II. In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxid Antioxid Med Sci. 2014; 3(3): 225-229. doi:10.5455/oams.191214.or.079


Web Style

Adewale OB, Onasanya A, Fadaka AO, Iwere H, Anadozie SO, Osukoya OA, Olayide II. In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. http://www.ejmoams.com/?mno=173781 [Access: May 22, 2018]. doi:10.5455/oams.191214.or.079


AMA (American Medical Association) Style

Adewale OB, Onasanya A, Fadaka AO, Iwere H, Anadozie SO, Osukoya OA, Olayide II. In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxid Antioxid Med Sci. 2014; 3(3): 225-229. doi:10.5455/oams.191214.or.079



Vancouver/ICMJE Style

Adewale OB, Onasanya A, Fadaka AO, Iwere H, Anadozie SO, Osukoya OA, Olayide II. In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxid Antioxid Med Sci. (2014), [cited May 22, 2018]; 3(3): 225-229. doi:10.5455/oams.191214.or.079



Harvard Style

Adewale, O. B., Onasanya, A., Fadaka, A. O., Iwere, H., Anadozie, S. O., Osukoya, O. A. & Olayide, I. I. (2014) In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxid Antioxid Med Sci, 3 (3), 225-229. doi:10.5455/oams.191214.or.079



Turabian Style

Adewale, Olusola B., Amos Onasanya, Adewale O. Fadaka, Hilda Iwere, Scholastica O. Anadozie, Olukemi A. Osukoya, and Israel I. Olayide. 2014. In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxidants and Antioxidants in Medical Science, 3 (3), 225-229. doi:10.5455/oams.191214.or.079



Chicago Style

Adewale, Olusola B., Amos Onasanya, Adewale O. Fadaka, Hilda Iwere, Scholastica O. Anadozie, Olukemi A. Osukoya, and Israel I. Olayide. "In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain." Oxidants and Antioxidants in Medical Science 3 (2014), 225-229. doi:10.5455/oams.191214.or.079



MLA (The Modern Language Association) Style

Adewale, Olusola B., Amos Onasanya, Adewale O. Fadaka, Hilda Iwere, Scholastica O. Anadozie, Olukemi A. Osukoya, and Israel I. Olayide. "In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain." Oxidants and Antioxidants in Medical Science 3.3 (2014), 225-229. Print. doi:10.5455/oams.191214.or.079



APA (American Psychological Association) Style

Adewale, O. B., Onasanya, A., Fadaka, A. O., Iwere, H., Anadozie, S. O., Osukoya, O. A. & Olayide, I. I. (2014) In vitro antioxidant effect of aqueous extract of Solanum macrocarpon leaves in rat liver and brain. Oxidants and Antioxidants in Medical Science, 3 (3), 225-229. doi:10.5455/oams.191214.or.079





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