ISSN 2146-8389
 

Original Research (Original Article) 


Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells

Ozlem Erol, Nazli Arda, Gunhan Erdem.

Abstract
Objective: Oxidative DNA damages occur in the cells constantly exposed to reactive oxygen species that can originate from normal metabolic processes and from environmental agents. Accumulation of oxidative DNA damages has been observed in several pathologies, such as aging, carcinogenesis and degenerative diseases. In this study the hypothesis that gallic acid, one of the most distributed phenolics in plants, could prevent the H2O2-induced both mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) damage was investigated.
Methods: The cells were pretreated with gallic acid (28 g/ml) for 4 h before the induction of oxidative stress by H2O2 (300 M) exposure for 1 h. DNA damage was assessed in the mitochondrial DNA and two nuclear regions using quantitative polymerase chain reaction (qPCR) assay.
Results: Pretreatment with gallic acid significantly reduced both nDNA and mtDNA damages occurred with H2O2 exposure.
Conclusion: The results clearly demonstrate that gallic acid has a protective effect against oxidative damage for both nDNA and mtDNA in HeLa cells. Gallic acid is most likely to act as an antimutagenic/anticarcinogenic agent through the protection of genome against the damaging effect of chronic oxidative stress.

Key words: DNA damage, gallic acid, HeLa, qPCR


 
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How to Cite this Article
Pubmed Style

Erol O, Arda N, Erdem G. Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxid Antioxid Med Sci. 2015; 4(1): 28-32. doi:10.5455/oams.020315.or.081


Web Style

Erol O, Arda N, Erdem G. Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. http://www.ejmoams.com/?mno=172663 [Access: October 16, 2018]. doi:10.5455/oams.020315.or.081


AMA (American Medical Association) Style

Erol O, Arda N, Erdem G. Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxid Antioxid Med Sci. 2015; 4(1): 28-32. doi:10.5455/oams.020315.or.081



Vancouver/ICMJE Style

Erol O, Arda N, Erdem G. Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxid Antioxid Med Sci. (2015), [cited October 16, 2018]; 4(1): 28-32. doi:10.5455/oams.020315.or.081



Harvard Style

Erol, O., Arda, N. & Erdem, G. (2015) Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxid Antioxid Med Sci, 4 (1), 28-32. doi:10.5455/oams.020315.or.081



Turabian Style

Erol, Ozlem, Nazli Arda, and Gunhan Erdem. 2015. Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxidants and Antioxidants in Medical Science, 4 (1), 28-32. doi:10.5455/oams.020315.or.081



Chicago Style

Erol, Ozlem, Nazli Arda, and Gunhan Erdem. "Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells." Oxidants and Antioxidants in Medical Science 4 (2015), 28-32. doi:10.5455/oams.020315.or.081



MLA (The Modern Language Association) Style

Erol, Ozlem, Nazli Arda, and Gunhan Erdem. "Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells." Oxidants and Antioxidants in Medical Science 4.1 (2015), 28-32. Print. doi:10.5455/oams.020315.or.081



APA (American Psychological Association) Style

Erol, O., Arda, N. & Erdem, G. (2015) Protective response of gallic acid from oxidative nuclear and mitochondrial DNA damage in HeLa cells. Oxidants and Antioxidants in Medical Science, 4 (1), 28-32. doi:10.5455/oams.020315.or.081





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