ISSN 2146-8389
 

Original Research (Original Article) 


Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats

Roshni Satishkumar Patel, Priyanshee Gohil.

Cited by (1)

Abstract
Objective: Cerebral ischemia induced by stroke is one of the major causes of death and disability in the world and reactive oxygen species play key role in pathogenesis of cerebral ischemia. Chemical structure of embelin is similar to antioxidant çoenzyme Q10 and embelin is reported to have potent antioxidant activity. Therefore, the present investigation is carried out to study the effect of embelin using the middle cerebral artery occlusion (MCAO)-induced focal cerebral ischemia model.
Methods: Male Wistar rats were treated with embelin (50, 75 and 100 mg/kg, p.o.) for 20 days, followed by MCAO-induced focal cerebral ischemia. Measurement of neurological score, infarct size and malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in brain homogenates were carried out at the end of study.
Results: Embelin decreases MDA level whereas increases SOD and CAT level as compared to ischemic control group. Moreover, embelin decreases infarct size, but there was no improvement in neurological score.
Conclusion: Pretreatment of embelin enhances the antioxidant defense and thereby ameliorates cerebral ischemia/reperfusion induced injury in the rat MCAO model.

Key words: Antioxidant; Cerebral ischemia; Embelin; MCAO


 
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REFERENCES
1. Zhang Y, Chapman AM, Plested M, Jackson D, Purroy F. The incidence, prevalence, and mortality of stroke in France, Germany, Italy, Spain, the UK, and the US: a literature review. Stroke Res Treat 2012; 2012:436125.
2. Woodruff TM, Thundyil J, Tang SC, Sobey CG, Taylor SM, Arumugam TV. Pathophysiology, treatment, and animal and cellular models of human ischemic stroke. Mol Neurodegener. 2011;6:11. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
3. Candelario-Jalil E, Ajamieh HH, Sam S, Martinez, G Leon, Fernandez OS. Nimesulide limits kainate-induced oxidative damage in the rat hippocampus. Eur J Pharmacol 2000; 3:295-8. [DOI via Crossref]   
4. Candelario-Jalil E, Mhadu NH, Al-Dalain SM, Martinez G, Leon OS. Time course of oxidative damage in different brain regions following transient cerebral ischemia in gerbils. Neurosci Res 2001; 3:233-41. [DOI via Crossref]   
5. Ikeda S, Harada K, Ohwatashi A, Kamikawa Y. Effects of edaravone, a free radical scavenger, on photochemically induced cerebral infarction in a rat hemiplegic model. ScientificWorldJournal 2013; 2013:175280. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
6. Margaill I, Plotkine M, Lerouet D. Antioxidant strategies in the treatment of stroke. Free Radic Biol Med 2005; 4:429-43. [DOI via Crossref]    [Pubmed]   
7. Kim H. Neuroprotective herbs for stroke therapy in traditional eastern medicine. Neurol Res 2005; 3:287-301. [DOI via Crossref]    [Pubmed]   
8. Wang NL, Liou YL, Lin MT, Lin CL, Chang CK. Chinese herbal medicine, Shengmai San, is effective for improving circulatory shock and oxidative damage in the brain during heatstroke. J Pharmacol Sci 2005; 2:253-65. [DOI via Crossref]   
9. Nazam Ansari M, Bhandari U, Islam F, Tripathi CD. Evaluation of antioxidant and neuroprotective effect of ethanolic extract of Embelia ribes Burm in focal cerebral ischemia/reperfusion-induced oxidative stress in rats. Fundam Clin Pharmacol 2008; 3:305-14. [DOI via Crossref]    [Pubmed]   
10. Bhandari Uma AMN. Protective effect of aqueous extract of Embelia ribes Burm fruits in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Indian J Pharmacol 2008; 5:215-20. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
11. Chauhan SK, Singh BP Agarwal S. A TLC identification and spectrophotometric estimation of embelin in Embelia ribes. Anc Sci Life 1999; 1-2:46-8.
12. Bhandari U, Jain N, Pillai KK. Further studies on antioxidant potential and protection of pancreatic beta-cells by Embelia ribes in experimental diabetes. Exp Diabetes Res 2007; 2007:15803. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
13. Dixit VP, Bhargava SK. Reversible contraception like activity of embelin in male dogs (Canis indicus Linn). Andrologia 1983; 5:486-94.
14. Gupta OP, Ali MM, Ray Ghatak BJ, Atal CK. Some pharmacological investigations of embelin and its semisynthetic derivatives. Indian J Physiol Pharmacol 1977; 1:31-9.
15. Chitra M, Devi CS, Sukumar E. Antibacterial activity of embelin. Fitoterapia 2003; 4:401-3. [DOI via Crossref]   
16. Chander H, Ahmed SM. Comparative evaluation of fungicidal quinones and natural embelin against some insect pests of storage. J Stored Prod Res 1989; 2:87-91. [DOI via Crossref]   
17. Chitra M, Sukumar E, Devi CS. [3H]-thymidine uptake and lipid peroxidation by tumor cells on embelin treatment: an in vitro study. Oncology 1995; 1:66-8. [DOI via Crossref]   
18. Bhandari U, Kanojia R, Pillai KK. Effect of ethanolic extract of Embelia ribes on dyslipidemia in diabetic rats. Int J Exp Diabetes Res 2002; 3:159-62. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
19. Bhandari U, Ansari MN, Islam F. Cardioprotective effect of aqueous extract of Embelia ribes Burm fruits against isoproterenol-induced myocardial infarction in albino rats. Indian J Exp Biol 2008; 1:35-40.
20. Ostrowski RP. Effect of coenzyme Q(10) on biochemical and morphological changes in experimental ischemia in the rat brain. Brain Res Bull 2000; 4:399-407. [DOI via Crossref]   
21. Matthews RT, Yang L, Browne S, Baik M, Beal MF. Coenzyme Q10 administration increases brain mitochondrial concentrations and exerts neuroprotective effects. Proc Natl Acad Sci USA 1998; 15:8892-7. [DOI via Crossref]   
22. Belayev L, Alonso OF, Busto R, Zhao W, Ginsberg MD. Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model. Stroke 1996; 9:1616-22. [DOI via Crossref]   
23. Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989; 1:84-91. [DOI via Crossref]   
24. Bederson JB, Pitts LH, Tsuji M, Nishimura MC, Davis RL, Bartkowski H. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination. Stroke 1986; 3:472-6. [DOI via Crossref]   
25. Isayama K, Pitts LH, Nishimura MC. Evaluation of 2,3,5-triphenyltetrazolium chloride staining to delineate rat brain infarcts. Stroke 1991; 11:1394-8. [DOI via Crossref]   
26. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 2:351-8. [DOI via Crossref]   
27. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972; 10:3170-5.
28. Aebi HE. Catalase in vitro. Methods Enzymol 1984; 105:121-6. [DOI via Crossref]   
29. Belayev L, Busto R, Zhao W, Ginsberg MD. HU-211, a novel noncompetitive N-methyl-D-aspartate antagonist, improves neurological deficit and reduces infarct volume after reversible focal cerebral ischemia in the rat. Stroke 1995; 12:2313-9. [DOI via Crossref]   
30. Yousuf S, Salim S, Ahmad M, Ahmed AS, Ansari MA, Islam F. Protective effect of Khamira Abresham Uood Mastagiwala against free radical induced damage in focal cerebral ischemia. J Ethnopharmacol 2005; 2:179-84. [DOI via Crossref]    [Pubmed]   
31. Bederson JB, Pitts LH, Germano SM, Nishimura MC, Davis RL, Bartkowski HM. Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke 1986; 6:1304-8. [DOI via Crossref]   
32. Ishibashi S, Kuroiwa T, Katsumata N, Yuan SL, Endo S, Mizusawa H. Extrapyramidal motor symptoms versus striatal infarction volume after focal ischemia in Mongolian gerbils. Neuroscience 2004; 2:269-75. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

Plant Derived Phytocompound, Embelin in CNS Disorders: A Systematic Review
Front. Pharmacol. 2017; 8(): .

1
 
How to Cite this Article
Pubmed Style

Roshni Satishkumar Patel, Priyanshee Gohil. Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxid Antioxid Med Sci. 2014; 3(2): 135-139. doi:10.5455/oams.020714.or.068


Web Style

Roshni Satishkumar Patel, Priyanshee Gohil. Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. http://www.ejmoams.com/?mno=156578 [Access: November 16, 2018]. doi:10.5455/oams.020714.or.068


AMA (American Medical Association) Style

Roshni Satishkumar Patel, Priyanshee Gohil. Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxid Antioxid Med Sci. 2014; 3(2): 135-139. doi:10.5455/oams.020714.or.068



Vancouver/ICMJE Style

Roshni Satishkumar Patel, Priyanshee Gohil. Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxid Antioxid Med Sci. (2014), [cited November 16, 2018]; 3(2): 135-139. doi:10.5455/oams.020714.or.068



Harvard Style

Roshni Satishkumar Patel, Priyanshee Gohil (2014) Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxid Antioxid Med Sci, 3 (2), 135-139. doi:10.5455/oams.020714.or.068



Turabian Style

Roshni Satishkumar Patel, Priyanshee Gohil. 2014. Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxidants and Antioxidants in Medical Science, 3 (2), 135-139. doi:10.5455/oams.020714.or.068



Chicago Style

Roshni Satishkumar Patel, Priyanshee Gohil. "Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats." Oxidants and Antioxidants in Medical Science 3 (2014), 135-139. doi:10.5455/oams.020714.or.068



MLA (The Modern Language Association) Style

Roshni Satishkumar Patel, Priyanshee Gohil. "Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats." Oxidants and Antioxidants in Medical Science 3.2 (2014), 135-139. Print. doi:10.5455/oams.020714.or.068



APA (American Psychological Association) Style

Roshni Satishkumar Patel, Priyanshee Gohil (2014) Effect of embelin in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Oxidants and Antioxidants in Medical Science, 3 (2), 135-139. doi:10.5455/oams.020714.or.068





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