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Original Research (Original Article) Open Access


Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves

Vienna Saraswaty, Chandra Risdian, Raden A. A. Lelono, Tjandrawati Mozef.

Cited by (2)

Abstract
Objective: Artocarpus altilis or Sukun is one kind of herbal medicine that is usually used by Indonesian people. It is widely known that the flavonoid derivative of A.altilis, one of its major bioactive constituent, has a broad range of pharmacological activity such as antioxidant, antibacterial, anti-inflamantory, antidiabetic and many others. In this study we investigated the bioactive constituent and influence of ethanol concentration, time and temperature on the antioxidant and antibacterial activity of A.altilis leaves in order to find appropriate and effective condition for its extraction.
Methods: A.altilis leaves were extracted with different ethanol concentration (50, 60 and 70% v/v), time (1, 2, 3, 4 and 24 h) and temperature (70oC and room temperature). Influence of different extraction processes was analyzed on antioxidant capacity, antibacterial activity, total phenolic and total flavonoid content. Isolation of bioactive compound was performed by column chromatography method and structure was elucidated by 1H and 13C FT-NMR (Fourier Transform-Nuclear Magnetic Resonance) spectra.
Results: Concentration of 70% v/v ethanol, 70oC and 1 h extraction process were found to be the most appropriate condition for extraction of A.altilis leaves in order to obtain bioactive components which gave both antioxidant and antibacterial activity against E.coli while yield of total flavonoid and phenolic content of extract should not less than 1.9 and 1.8% w/w, respectively. Structure elucidation of isolated compound identified that prenylated flavonoid, i.e. 1-(2,4-dihydroxyphenyl)-3-(8-hydroxy-2-methyl-2-(4-methylpent-3-en-1-yl)-2H-chromen-5-yl)propa-1-one is one of its important bioactive constituent.
Conclusion: Use of ethanol concentration, temperature and time influenced the yield of total phenolic and flavonoid content and bioactivity of A.altilis extract. It is recommended to heat the extraction process to 70oC in order to obtain both optimal antioxidant and antibacterial activity from ethanol extract of A.altilis leaves.

Key words: Antibacterial, Artocarpus altilis, ethanol, temperature


 
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How to Cite this Article
Pubmed Style

Saraswaty V, Risdian C, Lelono RAA, Mozef T. Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxid Antioxid Med Sci. 2015; 4(2): 97-102. doi:10.5455/oams.240515.or.086


Web Style

Saraswaty V, Risdian C, Lelono RAA, Mozef T. Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. http://www.ejmoams.com/?mno=1250 [Access: February 20, 2018]. doi:10.5455/oams.240515.or.086


AMA (American Medical Association) Style

Saraswaty V, Risdian C, Lelono RAA, Mozef T. Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxid Antioxid Med Sci. 2015; 4(2): 97-102. doi:10.5455/oams.240515.or.086



Vancouver/ICMJE Style

Saraswaty V, Risdian C, Lelono RAA, Mozef T. Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxid Antioxid Med Sci. (2015), [cited February 20, 2018]; 4(2): 97-102. doi:10.5455/oams.240515.or.086



Harvard Style

Saraswaty, V., Risdian, C., Lelono, R. A. A. & Mozef, T. (2015) Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxid Antioxid Med Sci, 4 (2), 97-102. doi:10.5455/oams.240515.or.086



Turabian Style

Saraswaty, Vienna, Chandra Risdian, Raden A. A. Lelono, and Tjandrawati Mozef. 2015. Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxidants and Antioxidants in Medical Science, 4 (2), 97-102. doi:10.5455/oams.240515.or.086



Chicago Style

Saraswaty, Vienna, Chandra Risdian, Raden A. A. Lelono, and Tjandrawati Mozef. "Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves." Oxidants and Antioxidants in Medical Science 4 (2015), 97-102. doi:10.5455/oams.240515.or.086



MLA (The Modern Language Association) Style

Saraswaty, Vienna, Chandra Risdian, Raden A. A. Lelono, and Tjandrawati Mozef. "Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves." Oxidants and Antioxidants in Medical Science 4.2 (2015), 97-102. Print. doi:10.5455/oams.240515.or.086



APA (American Psychological Association) Style

Saraswaty, V., Risdian, C., Lelono, R. A. A. & Mozef, T. (2015) Influence of ethanol concentration and temperature on antioxidant and antibacterial activity from Artocarpus altilis (Parkinson) Fosberg leaves. Oxidants and Antioxidants in Medical Science, 4 (2), 97-102. doi:10.5455/oams.240515.or.086





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